维甲酸对急性髓细胞白血病细胞生长因子受体的调节作用
Modulation of growth factor receptors on acute myeloblastic leukemia cells by retinoic acid.
作者信息
Tohda S, Minden M D
机构信息
First Department of Internal Medicine, Tokyo Medical and Dental University.
出版信息
Jpn J Cancer Res. 1994 Apr;85(4):378-83. doi: 10.1111/j.1349-7006.1994.tb02370.x.
The effects of retinoic acid (RA) on the proliferation of acute myeloblastic leukemia (AML) cells were studied. AML samples were divided into three groups. Namely, RA stimulated blast colony formation by AML samples in group A and inhibited that by the samples in group B, regardless of added growth factors. For the samples in group C, RA inhibited the colonies formed by granulocyte colony-stimulating factor (G-CSF) but stimulated those by granulocyte macrophage CSF (GM-CSF). To investigate the mechanism involved, the effects of RA on growth factor receptors on AML cells were examined by flow cytometry using fluorolabeled ligands. For the samples in groups A and B, RA affected neither G-CSF receptor (GR) nor GM-CSF receptor (GMR). For the samples in group C, exposure to 10(-7) M RA for 1 day clearly increased GMR, but did not affect GR. This finding supports the hypothesis that the increase of GMR is one of the causes of the stimulative effects of RA on cells cultured with GM-CSF in group C.
研究了维甲酸（RA）对急性髓性白血病（AML）细胞增殖的影响。AML样本被分为三组。具体而言，无论是否添加生长因子，RA刺激A组AML样本形成原始细胞集落，而抑制B组样本形成原始细胞集落。对于C组样本，RA抑制粒细胞集落刺激因子（G-CSF）形成的集落，但刺激粒细胞巨噬细胞集落刺激因子（GM-CSF）形成的集落。为了研究其中涉及的机制，使用荧光标记配体通过流式细胞术检测RA对AML细胞上生长因子受体的影响。对于A组和B组样本，RA既不影响G-CSF受体（GR）也不影响GM-CSF受体（GMR）。对于C组样本，暴露于10^(-7) M RA 1天明显增加GMR，但不影响GR。这一发现支持了以下假设：GMR的增加是RA对C组中用GM-CSF培养的细胞产生刺激作用的原因之一。
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