von Herbay A, de Groot H, Hegi U, Stremmel W, Strohmeyer G, Sies H
Department of Medicine, University of Düsseldorf, Germany.
J Hepatol. 1994 Jan;20(1):41-6.
The RRR-alpha-tocopherol (vitamin E) content in plasma from 46 patients with liver diseases and 23 healthy controls was determined by high performance liquid chromatography and electrochemical detection. Patients were divided into three groups: alcoholic liver diseases (n = 17; group A), hemochromatosis (n = 17; group B) and Wilson's disease (n = 12; group C). Lipid-standardized alpha-tocopherol levels were determined to neutralize differences due to hyperlipemia. The ratio of serum vitamin E to serum lipids (cholesterol, triglycerides, phospholipids) was highest in healthy controls and in patients in group A with cirrhosis and normal transaminases and bilirubin. Patients in group A with acute or chronic ethanol intoxication and high bilirubin levels had a 37% lower lipid-standardized vitamin E level than controls. Patients in group B with hemochromatosis, showing high serum iron (> 180 micrograms/dl), a low free iron binding capacity (< 8 mumol/l) and high ferritin-levels (< 450 micrograms/l), had a 34% lower vitamin E/lipid ratio than healthy controls. No significant lowering of the vitamin E/lipid ratio was observed in the other patients in group B. A significant decrease (37%) in the vitamin E/lipid ratio was only detectable in patients with Wilson's disease (group C) showing high free serum copper (> 10 micrograms/dl). The data support a role for free radicals in the pathogenesis of active liver diseases.
采用高效液相色谱法和电化学检测法测定了46例肝病患者及23名健康对照者血浆中RRR-α-生育酚（维生素E）的含量。患者被分为三组：酒精性肝病（n = 17；A组）、血色素沉着症（n = 17；B组）和威尔逊病（n = 12；C组）。测定了脂质标准化的α-生育酚水平，以中和高脂血症引起的差异。血清维生素E与血清脂质（胆固醇、甘油三酯、磷脂）的比值在健康对照者以及A组患有肝硬化且转氨酶和胆红素正常的患者中最高。A组患有急性或慢性乙醇中毒且胆红素水平高的患者，其脂质标准化维生素E水平比对照组低37%。B组患有血色素沉着症的患者，血清铁水平高（> 180微克/分升）、游离铁结合能力低（< 8微摩尔/升）且铁蛋白水平高（< 450微克/升），其维生素E/脂质比值比健康对照者低34%。B组的其他患者未观察到维生素E/脂质比值有显著降低。仅在威尔逊病（C组）且血清游离铜水平高（> 10微克/分升）的患者中检测到维生素E/脂质比值显著下降（37%）。这些数据支持自由基在活动性肝病发病机制中的作用。
