Lowenthal M, Shaughnessy M A, Harris K E, Grammer L C
Department of Medicine, Northwestern University Medical School, Chicago, IL 60611.
J Lab Clin Med. 1994 Jun;123(6):869-73.
The purpose of this study was to determine whether workers sensitized by one acid anhydride, trimellitic anhydride (TMA), would possibly react immunologically to two other acid anhydrides, phthalic anhydride (PA) or maleic anhydride (MA). We studied serum samples from four workers with TMA asthma and immunoglobin E (IgE) against TMA conjugated to human serum albumin (TM-HSA). In enzyme-linked immunosorbent assay (ELISA) cross-inhibition studies, TM-HSA inhibited IgE binding to TM-HSA, but when 100 times more P-HSA or M-HSA was used, no significant inhibition occurred. However, in ELISA studies of P-HSA and M-HSA, we saw binding of specific serum IgE. Finally, in passive transfer studies in rhesus monkeys with serum from an individual with antibodies to all three acid anhydrides, the following titers were obtained: TM-HSA (1:32), P-HSA (1:8), M-HSA (negative). We conclude that cross-inhibition studies may not be the best method for determining whether an individual sensitized to one antigen will react to a related antigen. The determination of biologic reactivity in a rhesus monkey model of passive cutaneous transfer makes it likely that biologic reactivity would also occur in a human sensitized to TMA and then exposed to another anhydride such as PA.
本研究的目的是确定被一种酸酐偏苯三酸酐（TMA）致敏的工人是否可能对另外两种酸酐邻苯二甲酸酐（PA）或马来酸酐（MA）产生免疫反应。我们研究了4名患有TMA哮喘的工人的血清样本以及针对与人血清白蛋白偶联的TMA的免疫球蛋白E（IgE）（TM-HSA）。在酶联免疫吸附测定（ELISA）交叉抑制研究中，TM-HSA抑制了IgE与TM-HSA的结合，但当使用的P-HSA或M-HSA量多100倍时，未出现明显抑制。然而，在对P-HSA和M-HSA的ELISA研究中，我们观察到了特异性血清IgE的结合。最后，在对具有针对所有三种酸酐抗体的个体的血清进行恒河猴被动转移研究中，获得了以下滴度：TM-HSA（1:32），P-HSA（1:8），M-HSA（阴性）。我们得出结论，交叉抑制研究可能不是确定对一种抗原致敏的个体是否会对相关抗原产生反应的最佳方法。在恒河猴被动皮肤转移模型中确定生物反应性表明，在对TMA致敏然后接触另一种酸酐（如PA）的人类中也可能发生生物反应。
