Medina-Sánchez M, Barneo-Serra L, Menendez-Peláez A, Martinez-Esteban M
Department of Surgery, University of Oviedo Medical School, Spain.
J Lab Clin Med. 1994 Jun;123(6):921-9.
The effect of pancreatic islet transplantation on the development of diabetic myopathy in streptozotocin-induced diabetic Lewis rats was examined histochemically and morphometrically in a proximal striated (rectus femoris) muscle. Diabetes was induced by streptozotocin administration, and diabetic animals were transplanted by intraportal grafts 6 weeks later. Islet-transplanted rats returned to euglycemia usually within the first 24 hours after transplantation and remained euglycemic over the subsequent 12-week observation period. Transplanted animals were compared with age-matched nontransplanted diabetic rats and nondiabetic age-matched control rats. Successful isotransplantation completely prevented the characteristic fast twitch (type IIB, glycolytic) fiber atrophy and also the changes in the fiber-type relative percentages, with prevention of the significant increase in the frequency of slow twitch oxidative (type I) and fast oxidative/glycolytic (type IIA) fibers at the expense of fast twitch glycolytic (type IIB) fibers. The histochemical appearance of all fiber types studied from muscles in transplanted rats was identical to equivalent fibers in age-matched control rats. Our data suggest that diabetic muscle pathology could be reversed and the progression of diabetic amyotrophy halted through the restoration of a euglycemic state by successful pancreatic islet transplantation, at least in short-term experimental diabetes.
在链脲佐菌素诱导的糖尿病Lewis大鼠中，通过组织化学和形态测量学方法，研究了胰岛移植对近端横纹肌（股直肌）糖尿病性肌病发展的影响。通过给予链脲佐菌素诱导糖尿病，6周后对糖尿病动物进行门静脉内移植。胰岛移植大鼠通常在移植后的头24小时内恢复正常血糖，并在随后的12周观察期内保持正常血糖。将移植动物与年龄匹配的未移植糖尿病大鼠和非糖尿病年龄匹配对照大鼠进行比较。成功的同基因移植完全防止了特征性的快肌纤维（IIB型，糖酵解型）萎缩以及纤维类型相对百分比的变化，防止了以快肌糖酵解纤维（IIB型）为代价的慢肌氧化纤维（I型）和快肌氧化/糖酵解纤维（IIA型）频率的显著增加。从移植大鼠肌肉中研究的所有纤维类型的组织化学外观与年龄匹配对照大鼠的相应纤维相同。我们的数据表明，至少在短期实验性糖尿病中，通过成功的胰岛移植恢复正常血糖状态，可以逆转糖尿病肌肉病理并阻止糖尿病性肌萎缩的进展。
