二异丁香酚对血小板血栓素形成和磷酸肌醇分解的抑制作用。

Inhibition of platelet thromboxane formation and phosphoinositides breakdown by diisoeugenol.

作者信息

Lin C H, Kuo Y H, Lin Y L, Teng C M

机构信息

Pharmacological Institute, College of Medicine, National Taiwan University, Taipei.

出版信息

J Pharm Pharmacol. 1994 Jan;46(1):54-7. doi: 10.1111/j.2042-7158.1994.tb03720.x.

DOI:10.1111/j.2042-7158.1994.tb03720.x

PMID:8201527

Abstract

Diisoeugenol inhibited the platelet aggregation and ATP release of rabbit platelets caused by ADP, arachidonic acid, platelet-activating factor (PAF), collagen and thrombin. Prolongation of the incubation time of platelets with diisoeugenol did not cause further inhibition and the aggregability of platelets could not be restored after washing. In human platelet-rich plasma, diisoeugenol inhibited the biphasic aggregation and ATP release induced by adrenaline and ADP in a concentration-dependent manner. Thromboxane B2 formation caused by arachidonic acid, collagen and thrombin was markedly inhibited by diisoeugenol in a concentration-dependent manner. Diisoeugenol also inhibited the formation of inositol monophosphate caused by collagen, PAF and thrombin. The cAMP level of washed platelets was not changed by diisoeugenol. It is concluded that the antiplatelet effect of diisoeugenol is due to the inhibition of thromboxane formation and phosphoinositides breakdown.

摘要

二异丁香酚抑制由二磷酸腺苷（ADP）、花生四烯酸、血小板活化因子（PAF）、胶原蛋白和凝血酶引起的兔血小板聚集及ATP释放。用二异丁香酚延长血小板孵育时间不会导致进一步抑制，且洗涤后血小板的聚集能力无法恢复。在富含人血小板的血浆中，二异丁香酚以浓度依赖性方式抑制肾上腺素和ADP诱导的双相聚集及ATP释放。二异丁香酚以浓度依赖性方式显著抑制由花生四烯酸、胶原蛋白和凝血酶引起的血栓素B2形成。二异丁香酚还抑制由胶原蛋白、PAF和凝血酶引起的肌醇单磷酸形成。二异丁香酚不会改变洗涤后血小板的环磷酸腺苷（cAMP）水平。得出结论：二异丁香酚的抗血小板作用归因于对血栓素形成和磷酸肌醇分解的抑制。