Ko F N, Wu T S, Liou M J, Huang T F, Teng C M
Pharmacological Institute, College of Medicine, National Taiwan University, Taipei.
Thromb Haemost. 1989 Nov 24;62(3):996-9.
Osthole, isolated from Chinese herb Angelica pubescens, inhibited platelet aggregation and ATP release induced by ADP, arachidonic acid, PAF, collagen, ionophore A23187 and thrombin in washed rabbit platelets. It showed a weak activity in platelet-rich plasma. Osthole inhibited the thromboxane B2 formation caused by arachidonic acid, collagen, ionophore A23187 and thrombin in washed platelets, and also the thromboxane B2 formation caused by the incubation of lysed platelet homogenate with arachidonic acid. The generation of inositol phosphates in washed platelets caused by collagen, PAF and thrombin was suppressed by osthole. These data indicate that the inhibitory effect of osthole on platelet aggregation and release reaction was due to the inhibition of thromboxane formation and phosphoinositides breakdown.
从中药材独活中分离得到的蛇床子素，可抑制洗涤过的兔血小板中由二磷酸腺苷（ADP）、花生四烯酸、血小板活化因子（PAF）、胶原、离子载体A23187和凝血酶诱导的血小板聚集及ATP释放。在富血小板血浆中，它表现出较弱的活性。蛇床子素抑制洗涤过的血小板中由花生四烯酸、胶原、离子载体A23187和凝血酶引起的血栓素B2生成，以及裂解的血小板匀浆与花生四烯酸孵育所引起的血栓素B2生成。蛇床子素可抑制胶原、PAF和凝血酶引起的洗涤过的血小板中肌醇磷酸的生成。这些数据表明，蛇床子素对血小板聚集和释放反应的抑制作用是由于其对血栓素生成及磷酸肌醇分解的抑制。
