Ebisuno S, Koul H, Menon M, Scheid C
Division of Urology, Minami Wakayama National Hospital, Japan.
J Urol. 1994 Jul;152(1):237-42. doi: 10.1016/s0022-5347(17)32869-0.
The present studies examined oxalate handling in LLC-PK1 cells, an epithelial cell line of porcine origin. These cells appear to express transport systems for oxalate, as evidenced by the fact that uptake was saturable, time dependent and sensitive to the anion transport inhibitor DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid). Oxalate uptake in these cells was also affected by the presence of certain inorganic anions (Cl-, SO4(2-), or HCO3-) but not by organic anions (para-aminohippurate, urate, malate, phenylsuccinate, succinate). This uptake was Na independent and unaffected by changes in membrane potential but was affected by external pH, with acidic pH stimulating and alkaline pH inhibiting oxalate accumulation. These findings suggest that LLC-PK1 cells express oxalate transporters similar to those observed in the mammalian renal cortex. Further studies using these cells may prove useful in defining the conditions that govern transcellular oxalate flux in renal epithelial cells.
本研究检测了LLC-PK1细胞（一种源自猪的上皮细胞系）对草酸盐的处理情况。这些细胞似乎表达了草酸盐转运系统，摄取具有饱和性、时间依赖性且对阴离子转运抑制剂DIDS（4,4'-二异硫氰基芪-2,2'-二磺酸）敏感，这一事实证明了这一点。这些细胞对草酸盐的摄取也受到某些无机阴离子（Cl-、SO4(2-)或HCO3-）的存在的影响，但不受有机阴离子（对氨基马尿酸盐、尿酸盐、苹果酸盐、苯琥珀酸盐、琥珀酸盐）的影响。这种摄取不依赖于Na，不受膜电位变化的影响，但受外部pH值的影响，酸性pH值刺激草酸盐积累，碱性pH值抑制草酸盐积累。这些发现表明，LLC-PK1细胞表达的草酸盐转运体与在哺乳动物肾皮质中观察到的相似。使用这些细胞进行的进一步研究可能有助于确定控制肾上皮细胞跨细胞草酸盐通量的条件。
