Myers R B, Meredith R F, Schlom J, LoBuglio A F, Bueschen A J, Wheeler R H, Stockard C R, Grizzle W E
Department of Pathology, University of Alabama at Birmingham 35294-7331.
J Urol. 1994 Jul;152(1):243-6. doi: 10.1016/s0022-5347(17)32870-7.
We examined the expression of two well-characterized oncofetal antigens, the tumor associated glycoprotein-72 (TAG-72) and carcinoembryonic antigen (CEA), in malignant prostatic tissues. Three specific monoclonal antibodies, B72.3, CC49 and CC83, were used to examine the expression of TAG-72. Immunoreactivity was detected in 63% of the malignant specimens using B72.3. CC49 and CC83 were more sensitive than B72.3 in detecting TAG-72 expression. Immunoreactivity was detected in approximately 80% of prostatic adenocarcinomas with CC49 or CC83. The pattern and localization of TAG-72 immunoreactivity were similar for the three antibodies with most immunoreactivity observed within the cytoplasm of malignant cells and within the lumens of malignant glands. TAG-72 immunoreactivity was not detected within benign epithelium or stroma, with the exception of focal epithelial expression in areas of acute prostatitis. The COL-1 antibody to CEA did not detect CEA in benign glands, stroma, or malignant cells of prostate specimens resected for prostatic adenocarcinoma. These results demonstrate that TAG-72, but not CEA, is frequently expressed in prostatic adenocarcinomas.
我们检测了两种特征明确的癌胚抗原，即肿瘤相关糖蛋白72（TAG-72）和癌胚抗原（CEA）在前列腺恶性组织中的表达。使用三种特异性单克隆抗体B72.3、CC49和CC83检测TAG-72的表达。使用B72.3在63%的恶性标本中检测到免疫反应性。CC49和CC83在检测TAG-72表达方面比B72.3更敏感。使用CC49或CC83在大约80%的前列腺腺癌中检测到免疫反应性。三种抗体的TAG-72免疫反应性模式和定位相似，大多数免疫反应性出现在恶性细胞的细胞质内和恶性腺管腔内。除了急性前列腺炎区域的局灶性上皮表达外，在良性上皮或基质中未检测到TAG-72免疫反应性。用于检测CEA的COL-1抗体在因前列腺腺癌而切除的前列腺标本的良性腺管、基质或恶性细胞中未检测到CEA。这些结果表明，TAG-72而非CEA在前列腺腺癌中经常表达。
