4-二甲基氨基苯乙胺，一种灵敏、特异、可电化学检测的单胺氧化酶-B底物。

4-Dimethylaminophenethylamine, a sensitive, specific, electrochemically detectable monoamine oxidase-B substrate.

作者信息

Reyes-Parada M, Scorza M C, Silveira R, Dajas F, Cassels B K

机构信息

Cell Biology Division, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.

出版信息

Life Sci. 1994;54(25):1955-63. doi: 10.1016/0024-3205(94)90130-9.

DOI:10.1016/0024-3205(94)90130-9

PMID:8201844

Abstract

4-Dimethylaminophenethylamine (DMAPEA) was characterized as an MAO substrate. This compound was unaffected by MAO-A, while its oxidation by MAO-B was linear as a function of both time and enzyme concentration, with Km = 5.8 microM and Vmax = 21.2 pmol/min/mg protein, using a crude rat brain mitochondrial suspension as source of MAO. Both DMAPEA and its oxidation product, 4-dimethylaminophenylacetic acid (DMAPAA), can be detected electrochemically at 0.85 V. The high MAO-B affinity and selectivity of DMAPEA, together with its low oxidation potential, make this molecule a unique tool to determine MAO-B activity in a wide variety of tissue preparations using HPLC-ED.

摘要

4-二甲基氨基苯乙胺（DMAPEA）被鉴定为单胺氧化酶（MAO）的底物。该化合物不受MAO-A的影响，而其被MAO-B氧化时，作为时间和酶浓度的函数呈线性关系，以粗制大鼠脑线粒体悬浮液作为MAO的来源时，其米氏常数（Km）= 5.8微摩尔，最大反应速度（Vmax）= 21.2皮摩尔/分钟/毫克蛋白质。DMAPEA及其氧化产物4-二甲基氨基苯乙酸（DMAPAA）均可在0.85伏的电压下通过电化学方法检测到。DMAPEA对MAO-B具有高亲和力和选择性，同时其氧化电位较低，这使得该分子成为一种独特的工具，可用于使用高效液相色谱-电化学检测法（HPLC-ED）在各种组织制剂中测定MAO-B的活性。