Portnoy B L, Goepfert J M, Harmon S M
Am J Epidemiol. 1976 Jun;103(6):589-94. doi: 10.1093/oxfordjournals.aje.a112263.
In an outbreak of gastrointestinal illness caused by consumption of home-grown raw vegetable sprouts contaminated by Bacillus cereus, victims developed symptoms after an incubation period of 6-15 hours. Four persons initially experienced nausea and vomiting, and this was followed in 3 cases by abdominal cramps and diarrhea. Bacteriologic investigation indicated that B. cereus on unsprouted seeds proliferated during germination in a commercially sold seed sprouting kit and reached levels in excess of 10(7) per gram. B. cereus isolated from the incriminated sprouts exhibited enterotoxigenic activity when tested by the ligated rabbit ileal loop technique, the dermal reaction in guinea pigs, and the rabbit skin capillary permeability test. The diversity of symptoms and incubation periods attributed to B. cereus requires analysis for this often overlooked organism whenever food-borne gastroenteritis is suspected.
在一次因食用被蜡样芽孢杆菌污染的自家种植的生豆芽而引发的胃肠道疾病暴发中,受害者在6至15小时的潜伏期后出现症状。4人最初出现恶心和呕吐,其中3例随后出现腹部绞痛和腹泻。细菌学调查表明,未发芽种子上的蜡样芽孢杆菌在商业销售的种子发芽套件中发芽过程中大量繁殖,每克含量超过10(7) 。通过结扎兔回肠袢技术、豚鼠皮肤反应和兔皮肤毛细血管通透性试验检测,从受污染豆芽中分离出的蜡样芽孢杆菌表现出产肠毒素活性。每当怀疑食源性肠胃炎时,对于这种经常被忽视的病原体,需要分析蜡样芽孢杆菌所致症状和潜伏期的多样性。