Mottet N K, Body R L
Am J Pathol. 1976 Jul;84(1):93-110.
The effects of methylmercury on the intestinal epithelium were studied in 14 adolescent male Macaca mulatta monkeys weighing 3 to 5 kg. They were divided into three groups: two controls received daily applesauce vehicle without methylmercury. Nine chronic low-dose animals received 0.2 to 1.0 mg of methylmercury per day for 80 to 491 days. Three acute high-dose animals received 2.0 mg methylmercury for 17 to 18 days, when they became terminally ill. Light and electron microscopic observations were made on samples of duodenum and ileum following perfusion and immersion fixation in a glutaraldehyde-paraformaldehyde fixative. Numerous uniquely structured inclusions were prominent in the Paneth cells of the chronic low-dose animals and some necrotic Paneth cells were seen, especially in the most chronic and higher dosed animals of the group. Acute high-dose treatment produced some inclusions in the Paneth cells similar to those of the chronic low-dose group, but degenerative and necrotic cells were more frequently seen. These alterations were not seen in other intestinal epithelial cells. Paneth cells are selectively altered. These findings suggest that a function of Paneth cells may be to eliminate metals from the body.
在14只体重3至5千克的青春期雄性恒河猴身上研究了甲基汞对肠道上皮的影响。它们被分为三组：两个对照组每天接受不含甲基汞的苹果酱赋形剂。九只慢性低剂量组动物每天接受0.2至1.0毫克甲基汞，持续80至491天。三只急性高剂量组动物接受2.0毫克甲基汞，持续17至18天，直至它们病入膏肓。在经戊二醛-多聚甲醛固定液灌注和浸泡固定后，对十二指肠和回肠样本进行了光镜和电镜观察。在慢性低剂量组动物的潘氏细胞中可见大量结构独特的包涵体，并且可见一些坏死的潘氏细胞，尤其是该组中病程最长和剂量最高的动物。急性高剂量处理在潘氏细胞中产生了一些与慢性低剂量组相似的包涵体，但可见更多的变性和坏死细胞。这些改变在其他肠道上皮细胞中未见。潘氏细胞被选择性改变。这些发现表明，潘氏细胞的一个功能可能是从体内清除金属。
