Gitlin J D, Gitlin J I, Gitlin D
Am J Physiol. 1976 Jun;230(6):1594-1602. doi: 10.1152/ajplegacy.1976.230.6.1594.
Specific plasma proteins were labeled with 131I, and their half-lives in lactating and nonlactating mice were determined. The proteins included mouse IgF and IgM, mouse, bovine, rabbit, and human IgG, human serum and salivary IgA, human transferrin and albumin, and mouse and human immunoglobin light chains. The rates at which these proteins are transferred across the mammary gland in mice suckling their young were calculated: transmammary protein transfer was found to be highly selective. The G-class immunoglobulins readily traversed the mammary gland, the rate being dependent on the IgG load presented to the transport system. Immunoglobulin lambda-chains and human serum IgA crossed the mammary gland even more rapidly than did the G immunoglobulins. Human IgA without secretory piece was transported into milk more rapidly than IgA with secretory piece attached. Protein transport across the mouse mammary gland was found to be similar to and yet different from protein transport across either the suckling mouse intestine or the mouse placenta as to which proteins crossed and at what relative rates.
用¹³¹I标记特定的血浆蛋白，并测定其在泌乳和非泌乳小鼠体内的半衰期。这些蛋白质包括小鼠IgF和IgM、小鼠、牛、兔和人IgG、人血清和唾液IgA、人转铁蛋白和白蛋白，以及小鼠和人免疫球蛋白轻链。计算了这些蛋白质在哺乳幼崽的小鼠中穿过乳腺的速率：发现跨乳腺蛋白转运具有高度选择性。G类免疫球蛋白很容易穿过乳腺，其速率取决于输送到转运系统的IgG负荷。免疫球蛋白λ链和人血清IgA穿过乳腺的速度甚至比G免疫球蛋白还要快。没有分泌片的人IgA比附着有分泌片的IgA更快地转运到乳汁中。发现蛋白质穿过小鼠乳腺的情况与穿过哺乳小鼠肠道或小鼠胎盘的蛋白质转运情况既有相似之处，也有不同之处，具体体现在哪些蛋白质能够穿过以及以何种相对速率穿过。
