用于乙炔还原测定的改进型制备豆科根瘤菌厌氧类菌体悬浮液的方法。
Improved method for preparing anaerobic bacteroid suspensions of Rhizobium leguminosarum for the acetylene reduction assay.
作者信息
Van Straten J, Roelofsen W
出版信息
Appl Environ Microbiol. 1976 Jun;31(6):859-63. doi: 10.1128/aem.31.6.859-863.1976.
Abstract
A method using ethylenediaminetetraacetate (EDTA) and toluene-treated pea bacteroid suspensions for the acetylene reduction assay is described. The high level of acetylene reduction by these bacteroids is comparable to that of intact plants. Reproducibility of the EDTA-toluene treatment is, on the average, within 5%. Preliminary experiments with soybeans indicate that the EDTA-toluene method might be applicable to other legumes as well.
摘要
本文描述了一种使用乙二胺四乙酸（EDTA）和甲苯处理豌豆类菌体悬浮液进行乙炔还原测定的方法。这些类菌体的高乙炔还原水平与完整植株相当。EDTA-甲苯处理的可重复性平均在5%以内。对大豆的初步实验表明，EDTA-甲苯法可能也适用于其他豆科植物。
相似文献
1
Improved method for preparing anaerobic bacteroid suspensions of Rhizobium leguminosarum for the acetylene reduction assay.用于乙炔还原测定的改进型制备豆科根瘤菌厌氧类菌体悬浮液的方法。
Appl Environ Microbiol. 1976 Jun;31(6):859-63. doi: 10.1128/aem.31.6.859-863.1976.
2
Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids.豆科根瘤菌类菌体中氨同化的酶
Can J Microbiol. 1975 Jul;21(7):1009-12. doi: 10.1139/m75-149.
3
Identification of the rhizobium strains in pea root nodules using genetic markers.利用遗传标记鉴定豌豆根瘤中的根瘤菌菌株。
J Gen Microbiol. 1975 Apr;87(2):343-50. doi: 10.1099/00221287-87-2-343.
4
Influence of ammonium chloride on the nitrogenase activity of nodulated pea plants (Pisum sativum).氯化铵对豌豆（Pisum sativum）根瘤植株固氮酶活性的影响。
Appl Environ Microbiol. 1978 Jun;35(6):1061-5. doi: 10.1128/aem.35.6.1061-1065.1978.
5
Respiration supported nitrogenase activity of isolated Rhizobium meliloti bacteroids.呼吸作用支持分离出的苜蓿根瘤菌类菌体的固氮酶活性。
J Cell Biochem. 1988 Sep;38(1):35-49. doi: 10.1002/jcb.240380105.
6
Action of water in depressing acetylene reduction by detached nodules.水分对离体根瘤乙炔还原作用的影响
Appl Microbiol. 1975 Mar;29(3):432-4. doi: 10.1128/am.29.3.432-434.1975.
7
Comparative response of Pisum sativum nodulated with indigenous soil Rhizobium populations and/or co-inoculated with a Rhizobium leguminosarum strain. I. Acetylene-reducing, dihydrogen- and carbon dioxide-evolving activities.用本地土壤根瘤菌群体结瘤的豌豆以及和豌豆根瘤菌菌株共接种的豌豆的比较反应。I. 乙炔还原、氢气和二氧化碳释放活性。
Folia Microbiol (Praha). 1991;36(3):271-6. doi: 10.1007/BF02814361.
8
Electron allocation to H+ and N2 by nitrogenase in Rhizobium leguminosarum bacteroids.豆科根瘤菌类菌体中固氮酶将电子分配给H⁺和N₂的过程。
Eur J Biochem. 1984 Jul 2;142(1):37-42. doi: 10.1111/j.1432-1033.1984.tb08247.x.
9
Oxygen requirement for acetylene reduction by pure cultures of rhizobia.根瘤菌纯培养物还原乙炔所需的氧量。
J Bacteriol. 1976 Jul;127(1):149-53. doi: 10.1128/jb.127.1.149-153.1976.
10
Ammonia assimilation by rhizobium cultures and bacteroids.根瘤菌培养物和类菌体对氨的同化作用。
J Gen Microbiol. 1975 Jan;86(1):39-48. doi: 10.1099/00221287-86-1-39.
引用本文的文献
1
Whole-Cell Hybridization of Frankia Strains with Fluorescence- or Digoxigenin-Labeled, 16S rRNA-Targeted Oligonucleotide Probes.弗兰克氏菌属菌株的全细胞杂交与荧光或地高辛标记的 16S rRNA 靶向寡核苷酸探针。
Appl Environ Microbiol. 1993 Jun;59(6):1709-16. doi: 10.1128/aem.59.6.1709-1716.1993.
2
Effect of Ammonium Chloride and Methionine Sulfoximine on the Acetylene Reduction of Detached Root Nodules of Peas (Pisum sativum).氯化铵和蛋氨酸亚砜对离体豌豆根瘤乙炔还原的影响。
Appl Environ Microbiol. 1979 Jan;37(1):73-9. doi: 10.1128/aem.37.1.73-79.1979.
3
Influence of ammonium chloride on the nitrogenase activity of nodulated pea plants (Pisum sativum).氯化铵对豌豆（Pisum sativum）根瘤植株固氮酶活性的影响。
Appl Environ Microbiol. 1978 Jun;35(6):1061-5. doi: 10.1128/aem.35.6.1061-1065.1978.
本文引用的文献
1
The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation.乙炔-乙烯法测定固氮作用中的 N2 固定：实验室和现场评价。
Plant Physiol. 1968 Aug;43(8):1185-207. doi: 10.1104/pp.43.8.1185.
2
Reactivation and hybridization of reduced alkaline phosphatase.还原碱性磷酸酶的再激活与杂交
Proc Natl Acad Sci U S A. 1962 Jul 15;48(7):1230-7. doi: 10.1073/pnas.48.7.1230.
3
A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA.乙二胺四乙酸（EDTA）导致大肠杆菌通透性非特异性增加。
Proc Natl Acad Sci U S A. 1965 Apr;53(4):745-50. doi: 10.1073/pnas.53.4.745.
4
ACTINOMYCIN SENSITIVITY IN ESCHERICHIA COLI PRODUCED BY EDTA.乙二胺四乙酸（EDTA）诱导大肠杆菌产生对放线菌素的敏感性
Biochem Biophys Res Commun. 1965 Jan 4;18:13-7. doi: 10.1016/0006-291x(65)90874-0.
5
Studies on the induction of beta-galactosidase in a cryptic strain of Escherichia coli.大肠杆菌隐性菌株中β-半乳糖苷酶诱导作用的研究。
Biochim Biophys Acta. 1959 Feb;31(2):525-38. doi: 10.1016/0006-3002(59)90029-0.
6
Some properties of nitrogen-fixing breis prepared from soybean root nodules.从大豆根瘤制备的固氮菌剂的一些特性。
Biochim Biophys Acta. 1966 Dec 28;130(2):304-12. doi: 10.1016/0304-4165(66)90225-x.
7
Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate.关于乙二胺四乙酸对大肠菌产生的通透性变化的研究。
J Biol Chem. 1968 May 10;243(9):2373-80.
8
The effect of ethylenediaminetetra-acetic acid on the cell walls of some gram-negative bacteria.乙二胺四乙酸对某些革兰氏阴性菌细胞壁的影响。
J Gen Microbiol. 1965 Jun;39(3):385-99. doi: 10.1099/00221287-39-3-385.
9
Estimation of nitrogenase in intact legumes.完整豆科植物中固氮酶的测定
Can J Microbiol. 1973 Feb;19(2):304-5. doi: 10.1139/m73-049.
Zotero 插件