Mitsuyama S, Tanaka S, Urushizaki F, Yoshida T, Ito A, Mori Y
Research Center, Taisho Pharmaceutical Co., Ltd., Saitama, Japan.
Res Commun Chem Pathol Pharmacol. 1994 Feb;83(2):179-93.
The effects of chondroitin polysulfate (CPS), a semi-synthetic oversulfated chondroitin sulfate, on the metabolism of extracellular matrix in human skin cells were examined. CPS accelerated the production of glycosaminoglycans (GAGs) in human dermal fibroblasts in a dose dependent manner (0.1-3.0 micrograms/ml), but did not modulate the production of noncollagenous protein and collagen, or [3H]-thymidine incorporation into DNA. However, higher concentrations (> 10 micrograms/ml) of CPS suppressed the [3H]-thymidine uptake by fibroblasts. More than 80% of total synthesized GAGs were found to be hyaluronate, whereas CPS did not alter their composition. These results indicated that CPS preferentially enhances the production of GAGs in human dermal fibroblasts, and that GAGs and DNA synthesis in the fibroblasts are independently regulated. Furthermore, CPS might be a unique material able to maintain the level of GAGs in the human skin.
研究了半合成的过度硫酸化硫酸软骨素——硫酸软骨素多糖（CPS）对人皮肤细胞外基质代谢的影响。CPS以剂量依赖性方式（0.1 - 3.0微克/毫升）加速人真皮成纤维细胞中糖胺聚糖（GAGs）的产生，但不调节非胶原蛋白和胶原蛋白的产生，也不影响[3H] - 胸苷掺入DNA。然而，较高浓度（>10微克/毫升）的CPS抑制成纤维细胞对[3H] - 胸苷的摄取。发现总合成GAGs的80%以上为透明质酸盐，而CPS并未改变其组成。这些结果表明，CPS优先增强人真皮成纤维细胞中GAGs的产生，并且成纤维细胞中GAGs和DNA的合成是独立调节的。此外，CPS可能是一种能够维持人皮肤中GAGs水平的独特物质。
