Nakamura T, Takagaki K, Shibata S, Tanaka K, Higuchi T, Endo M
Department of Biochemistry, Hirosaki University School of Medicine, Japan.
Biochem Biophys Res Commun. 1995 Mar 17;208(2):470-5. doi: 10.1006/bbrc.1995.1362.
The effects of xylosyl-beta-D-(4-methylumbelliferone) and its aglycone, 4-methylumbelliferone, on hyaluronic acid synthesis were investigated in cultured human skin fibroblasts. Xylosyl-beta-D-(4-methylumbelliferone) added to the medium of cultured cell reduced the synthesis of hyaluronic acid. Furthermore, 4-methylumbelliferone reduced the production of hyaluronic acid markedly. In addition, 4-methylumbelliferone had hardly any effect on proteoglycan synthesis, whereas xylosyl-beta-D-(4-methylumbelliferone) produced a large amount of glycosaminoglycan chains. The present results indicate that cells cultured with 4-methylumbelliferone produce a hyaluronic-acid-deficient extracellular matrix, which will be useful for functional studies of hyaluronic acid.
研究了木糖基-β-D-（4-甲基伞形酮）及其糖苷配基4-甲基伞形酮对培养的人皮肤成纤维细胞透明质酸合成的影响。添加到培养细胞培养基中的木糖基-β-D-（4-甲基伞形酮）降低了透明质酸的合成。此外，4-甲基伞形酮显著降低了透明质酸的产生。此外，4-甲基伞形酮对蛋白聚糖合成几乎没有影响，而木糖基-β-D-（4-甲基伞形酮）产生大量糖胺聚糖链。目前的结果表明，用4-甲基伞形酮培养的细胞产生缺乏透明质酸的细胞外基质，这将有助于透明质酸的功能研究。
