Artacho-Pérula E, Roldán-Villalobos R
Department of Morphological Sciences, University of Córdoba, Spain.
Res Exp Med (Berl). 1994;194(1):35-44. doi: 10.1007/BF02576364.
Recent developments in computer systems and new stereological tools have made estimation of quantitative data in biological material simple, easy and fast. The purpose of the present study is to show the advantages and disadvantages of a new program developed by the University of Córdoba for the determination of stereological measurements. As an example, endometrial material was used for demonstrating the technical characteristics. Thus, the nuclear area and volume-weighted mean nuclear volume-Vv(nucl)-of 30 routinely processed, paraffin-embedded endometrial specimens from 12 atypical hyperplasias (AH) and 18 well-differentiated adenocarcinomas (WDA) were investigated. We show the excellent visualization of the material and rapid measurement of these quantitative parameters using the Imago program. In addition, the mean values of Vv(nucl) are more efficient and useful in separating AH and WDA than the mean nuclear area. It is concluded that Vv(nucl) is a superior and sensitive estimator for discrimination.
计算机系统和新的体视学工具的最新发展使生物材料中定量数据的估计变得简单、容易且快速。本研究的目的是展示科尔多瓦大学开发的用于确定体视学测量的新程序的优缺点。作为示例，使用子宫内膜材料来展示技术特征。因此，对来自12例非典型增生（AH）和18例高分化腺癌（WDA）的30个常规处理的石蜡包埋子宫内膜标本的核面积和体积加权平均核体积-Vv(nucl)-进行了研究。我们展示了使用Imago程序对材料的出色可视化以及对这些定量参数的快速测量。此外，Vv(nucl)的平均值在区分AH和WDA方面比平均核面积更有效且有用。得出的结论是，Vv(nucl)是用于鉴别诊断的一种更优且敏感的估计指标。
