Petkova I, Koparanova O, Kemileva Z, Milanov S
Department of Pathophysiology, High Medical Institute, Sofia, Bulgaria.
Acta Physiol Pharmacol Bulg. 1993;19(3):61-5.
The aim of the study was to label TAM with radioactive iodine and to follow its distribution in different organs of rats. T-actemodulin is a thymic hormone isolated from calf thymus by a new technology. TAM was labeled according to the method of Greenwood and Hunter. Two major peaks were observed on the gel chromatography: The first peak contained the labeled TAM; the second peak contained 125I which was not bound to the organic compound. Additional purification of the first peak was performed on a Sephadex G-75 column, and the eluate was designated as purified peak I. After a radioimmune testing it could be concluded that TAM was labeled successfully and the peak I contained 125I-TAM. Peak I, purified peak I and the peak II were applied to young male Wistar rats. The radioactivity of pancreas, kidney, spleen, adrenals, liver, thymus, bone marrow, testis and thyroid were measured after killing the animals 10 min, 60 min, and 24 h after the injections. The pharmacokinetic study showed that all organs, except for the thyroid have a higher affinity for labeled TAM than for the free 125I. Higher accumulation of labeled TAM was observed in the pancreas, kidney, adrenals and spleen. A specific uptake of labeled TAM in these organs can be suggested. Functional interrelations between thymus and pancreas, spleen, adrenals are discussed.
本研究的目的是用放射性碘标记TAM，并追踪其在大鼠不同器官中的分布。T-actemodulin是一种通过新技术从小牛胸腺中分离出来的胸腺激素。TAM按照格林伍德和亨特的方法进行标记。在凝胶色谱上观察到两个主要峰：第一个峰含有标记的TAM；第二个峰含有未与有机化合物结合的125I。对第一个峰在Sephadex G-75柱上进行进一步纯化，洗脱液被指定为纯化峰I。经过放射免疫检测，可以得出TAM被成功标记且峰I含有125I-TAM的结论。将峰I、纯化峰I和峰II应用于年轻雄性Wistar大鼠。在注射后10分钟、60分钟和24小时处死动物后，测量胰腺、肾脏、脾脏、肾上腺、肝脏、胸腺、骨髓、睾丸和甲状腺的放射性。药代动力学研究表明，除甲状腺外，所有器官对标记的TAM的亲和力均高于对游离125I的亲和力。在胰腺、肾脏、肾上腺和脾脏中观察到标记的TAM有更高的蓄积。可以推测这些器官对标记的TAM有特异性摄取。讨论了胸腺与胰腺、脾脏、肾上腺之间的功能相互关系。
