Sugimoto Y, Namba T, Shigemoto R, Negishi M, Ichikawa A, Narumiya S
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Am J Physiol. 1994 May;266(5 Pt 2):F823-8. doi: 10.1152/ajprenal.1994.266.5.F823.
Distribution of the mRNAs for three subtypes of prostaglandin E (PGE) receptors in the mouse kidney was investigated by in situ hybridization. The mRNA for EP1 subtype, which is coupled to Ca2+ mobilization, was specifically localized to the collecting ducts from the cortex to the papilla. The mRNA for EP2 subtype, which is linked to stimulation of adenylate cyclase, was localized to the glomeruli. The mRNA for EP3 subtype, which is coupled to inhibition of adenylate cyclase, was located densely in the tubules in the outer medulla and in the distal tubules in the cortex. These results exhibit distinct cellular localization of three subtypes of PGE receptor in the kidney and suggest that PGE2 exerts multiple functions via these subtypes expressed in different segments of the nephron.
通过原位杂交研究了前列腺素E（PGE）受体三种亚型的mRNA在小鼠肾脏中的分布。与Ca2+动员偶联的EP1亚型的mRNA特异性定位于从皮质到乳头的集合管。与腺苷酸环化酶刺激相关的EP2亚型的mRNA定位于肾小球。与腺苷酸环化酶抑制偶联的EP3亚型的mRNA密集位于外髓质的肾小管和皮质的远端小管中。这些结果显示了PGE受体三种亚型在肾脏中的不同细胞定位，并表明PGE2通过在肾单位不同节段表达的这些亚型发挥多种功能。
