Guizani I, Van Eys G J, Ismail R B, Dellagi K
Laboratoire d'Hematologie et d'Immunopathologie, Faculte de Medecine de Tunis, Tunisia.
Am J Trop Med Hyg. 1994 May;50(5):632-40. doi: 10.4269/ajtmh.1994.50.632.
Recombinant DNA probes from a genomic Leishmania major library were screened for their potential to distinguish among Old World Leishmania taxa by Southern blot analysis. A probe, pDK10, was selected and tested on a panel of 58 Old World Leishmania strains that had already been typed isoenzymatically; these strains belong to the different species described so far and had been isolated from various hosts and vectors in 14 countries. In the present study, 45 zymodemes were represented. Using the pDK10 probe, we were able to differentiate between the different phenetic complexes. No variations in hybridization patterns were found within these complexes. In addition, there was a good concordance between identification based on DNA hybridization with the pDK10 probe and that based on isoenzyme typing. The probe has been applied in identifying Leishmania strains that were isolated in Tunisia from humans, animals, or insects. Our results show that the application of the pDK10 probe, in combination with a Pst I digestion of Leishmania DNA, could be a possible alternative to isoenzyme analysis for the identification of Leishmania strains.
通过Southern印迹分析，对来自硕大利什曼原虫基因组文库的重组DNA探针进行筛选，以评估其区分旧大陆利什曼原虫分类群的潜力。选择了一个探针pDK10，并在一组58株已通过同工酶法分型的旧大陆利什曼原虫菌株上进行测试；这些菌株属于目前已描述的不同物种，并且是从14个国家的各种宿主和媒介中分离得到的。在本研究中，代表了45个酶解模式。使用pDK10探针，我们能够区分不同的表型复合体。在这些复合体内未发现杂交模式的变化。此外，基于pDK10探针的DNA杂交鉴定与基于同工酶分型的鉴定之间具有良好的一致性。该探针已应用于鉴定在突尼斯从人、动物或昆虫中分离出的利什曼原虫菌株。我们的结果表明，将pDK10探针与利什曼原虫DNA的Pst I消化相结合应用，可能是替代同工酶分析用于鉴定利什曼原虫菌株的一种方法。
