Ryu J S, Min D Y, Shin M H, Cho Y H
Department of Parasitology, Hanyang University, College of Medicine, Seoul, Korea.
Korean J Parasitol. 1998 Sep;36(3):207-11. doi: 10.3347/kjp.1998.36.3.207.
In the present study, genomic DNAs were purified from Korean isolates (KT8, KT6, KT-Kim and KT-Lee) and foreign strains (CDC85, IR78 and NYH 286) of Trichomonas vaginalis, and hybridized with a probe based on the repetitive sequence cloned from T. vaginalis to observe the genetic differences. By Southern hybridization, all isolates of T. vaginalis except the NYH286 strain had 11 bands. Therefore all isolates examined were distinguishable into 3 groups according to their banding patterns; i) KT8, KT6 and KT-Kim isolates had 11 identical bands such as 1 kb, 1.2 kb, 1.6 kb, 1.9 kb, 2.3 kb, 2.7 kb, 3.2 kb, 3.4 kb, 3.8 kb, 4.9 kb and 6.0 kb. ii) The metronidazole-resistant IR78 strain had the same bands as KT-Lee isolate at bands of 1 kb, 1.2 kb, 1.6 kb, 1.8 kb, 2.1 kb, 2.5 kb, 2.7 kb, 2.9 kb, 3.4 kb, 5.0 kb and 6.0 kb. Bands of CDC85, metronidazole-resistant strain, were similar to those of IR78 and KT-Lee, except that 3.2 kb replaced 2.9 kb. iii) NYH286 particularly had 12 bands and band patterns were similar to IR78 with a few exceptions as follows: i) 6.2 kb in place of 6.0 kb, ii) 2.0 kb and 2.2 kb instead of 2.1 kb. Through the results obtained, genetic variance of T. vaginalis isolates was demonstrated by Southern hybridization.
在本研究中，从阴道毛滴虫的韩国分离株（KT8、KT6、KT-Kim和KT-Lee）以及国外菌株（CDC85、IR78和NYH 286）中纯化基因组DNA，并与基于从阴道毛滴虫克隆的重复序列的探针杂交，以观察遗传差异。通过Southern杂交，除NYH286菌株外，所有阴道毛滴虫分离株均有11条带。因此，根据其条带模式，所有检测的分离株可分为3组：i）KT8、KT6和KT-Kim分离株有11条相同的带，如1 kb、1.2 kb、1.6 kb、1.9 kb、2.3 kb、2.7 kb、3.2 kb、3.4 kb、3.8 kb、4.9 kb和6.0 kb。ii）耐甲硝唑的IR78菌株在1 kb、1.2 kb、1.6 kb、1.8 kb、2.1 kb、2.5 kb、2.7 kb、2.9 kb、3.4 kb、5.0 kb和6.0 kb的条带处与KT-Lee分离株具有相同的条带。耐甲硝唑菌株CDC85的条带与IR78和KT-Lee的条带相似，不同之处在于3.2 kb取代了2.9 kb。iii）NYH286特别有12条带，条带模式与IR78相似，但有以下几个例外：i）6.2 kb代替6.0 kb，ii）2.0 kb和2.2 kb代替2.1 kb。通过获得的结果，Southern杂交证明了阴道毛滴虫分离株的遗传变异。
