Suzuki S, Kominami R, Takada T, Yabusaki Y, Tanaka N, Tanaka S, Aizawa K, Suzuki T, Tanaka O, Hatakeyama K
First Dept. of Surgery, Niigata University School of Medicine.
Gan To Kagaku Ryoho. 1994 May;21 Suppl 1:52-7.
The mutagenicity of quercetin, a flavonoid, was examined by means of DNA fingerprint analysis using the Pc-1 and Pc-2 minisatellite probes that efficiently detect mutations due to recombination. Treatment of FM3A and BMT-11 tumor cells with 55 microM quercetin resulted in gain and loss of bands in the fingerprints in both cell lines. The frequencies of the clones having undergone mutation were 9/26 and 2/11, using Pc-1 probe, respectively, in the two lines. These results seem to provide a molecular basis for the phenotypic variations of BMT-11 tumor cells induced by quercetin, giving direct evidence of genetic instability of the tumor cells. Moreover, we examined for a possible correlation between frequencies of DNA recombinational mutations and cancer malignancy in human colon cancers. DNA of four human colon cancer tissues and corresponding peripheral blood cells were prepared, respectively, and examined by DNA fingerprint analysis using hPc-1 polymorphic minisatellite probe. These four specimens exhibited no extra-bands resulting from recombination and/or DNA slippage at present. We would explain how the prognosis of cancer patients is related to frequencies of DNA recombinational mutation.
通过使用能有效检测重组引起突变的Pc-1和Pc-2小卫星探针进行DNA指纹分析，研究了类黄酮槲皮素的致突变性。用55微摩尔槲皮素处理FM3A和BMT-11肿瘤细胞，导致两种细胞系指纹图谱中条带的增加和缺失。使用Pc-1探针时，两种细胞系中发生突变的克隆频率分别为9/26和2/11。这些结果似乎为槲皮素诱导的BMT-11肿瘤细胞的表型变异提供了分子基础，直接证明了肿瘤细胞的遗传不稳定性。此外，我们研究了人类结肠癌中DNA重组突变频率与癌症恶性程度之间的可能相关性。分别制备了四个人类结肠癌组织和相应外周血细胞的DNA，并使用hPc-1多态性小卫星探针通过DNA指纹分析进行检测。目前，这四个标本未表现出由重组和/或DNA滑动产生的额外条带。我们将解释癌症患者的预后如何与DNA重组突变频率相关。
