Suzuki S, Takada T, Sugawara Y, Muto T, Kominami R
First Department of Biochemistry, Niigata University School of Medicine.
Jpn J Cancer Res. 1991 Oct;82(10):1061-4. doi: 10.1111/j.1349-7006.1991.tb01757.x.
Quercetin, a flavonoid, is found in many fruits and vegetables. This drug was previously shown to affect the metastatic potential of mouse tumor cells. Mutagenicity of quercetin was examined by means of DNA fingerprint analysis using the Pc-1 probe that efficiently detects mutations due to recombination. Treatment of BMT-11 and FM3A tumor cells with 55 microM quercetin resulted in gain and loss of bands in the fingerprints in both cell lines. The frequencies of the clones having undergone mutation were 3/11 and 6/26, respectively. This suggests that quercetin is mutagenic and induces recombination. This result seems to provide a molecular basis for the phenotypic variations of BMT-11 tumor cells induced by quercetin.
槲皮素是一种类黄酮，存在于许多水果和蔬菜中。此前已证明这种药物会影响小鼠肿瘤细胞的转移潜能。使用能有效检测重组引起的突变的Pc - 1探针，通过DNA指纹分析检测了槲皮素的致突变性。用55微摩尔的槲皮素处理BMT - 11和FM3A肿瘤细胞，导致两个细胞系的指纹图谱中条带出现增减。发生突变的克隆频率分别为3/11和6/26。这表明槲皮素具有致突变性并诱导重组。这一结果似乎为槲皮素诱导的BMT - 11肿瘤细胞的表型变异提供了分子基础。
