Piña A L, Ormsby C E, Bermúdez-Rattoni F
Instituto de Fisiología Celular, UNAM, México, D.F.
Behav Neural Biol. 1994 Mar;61(2):196-201. doi: 10.1016/s0163-1047(05)80076-7.
Four groups of male Wistar rats showing disrupted inhibitory avoidance conditioning due to striatal lesions were studied. Three groups received striatal, cortical, or ventral mesencephalic brain grafts and the fourth group remained as a lesioned control. Sixty days postgraft the animals were retrained in an inhibitory avoidance task. The striatal-grafted animals were the only group that significantly improved in the ability to acquire the inhibitory avoidance task. Acetylcholinesterase histochemistry revealed positive patches of cells in the striatal grafts. Cortical grafts showed less reactivity, without patches. Immunocytochemical analyses for tyrosine hydroxylase revealed positive cell reactivity in the mesencephalic grafts and few positive fibers were detected in the border between the striatal grafts and the host tissue. These results demonstrate that striatal but not cortical or mesencephalic brain grafts can promote the restoration of the ability to acquire an inhibitory avoidance task and suggest that the acetylcholine tissue content is involved in the behavioral recovery.
对四组因纹状体损伤而表现出抑制性回避条件反射破坏的雄性Wistar大鼠进行了研究。三组接受了纹状体、皮质或腹侧中脑脑移植，第四组作为损伤对照组。移植后60天，对动物进行抑制性回避任务的再训练。纹状体移植动物是唯一在获得抑制性回避任务能力上有显著改善的组。乙酰胆碱酯酶组织化学显示纹状体移植物中有阳性细胞斑块。皮质移植物反应性较低，无斑块。酪氨酸羟化酶的免疫细胞化学分析显示中脑移植物中有阳性细胞反应，在纹状体移植物与宿主组织的边界处检测到少量阳性纤维。这些结果表明，纹状体脑移植而非皮质或中脑脑移植可以促进获得抑制性回避任务能力的恢复，并提示乙酰胆碱组织含量与行为恢复有关。
