通过注射依地酸钙钠对血清多巴胺-β-羟化酶进行体内抑制。

In vivo inhibition of serum dopamine-beta-hydroxylase by CaNa2 EDTA injection.

De Paris P, Caroldi S

Istituto di Medicina del Lavoro, University of Padova, Italy.

Hum Exp Toxicol. 1994 Apr;13(4):253-6. doi: 10.1177/096032719401300405.

Abstract

In humans CaNa2EDTA increases urinary excretion of lead as well as that of essential metals such as zinc, ferrum and manganese but not that of copper. 2. We studied the effect of CaNa2EDTA injection on serum dopamine-beta-hydroxylase, a copper-dependent enzyme, in three male lead welders hospitalized for suspected lead poisoning. 3. Injection of CaNa2EDTA (1000 mg i.v.) resulted in rapid reduction of serum dopamine-beta-hydroxylase activity which returned to normal level within 24 hours. 4. Copper sulphate (8 microns) but not ethylmaleimide (10 mM) restored in vitro serum dopamine-beta-hydroxylase activity to pre-dosing values which indicates that CaNa2EDTA inhibits dopamine-beta-hydroxylase by removing copper from the enzyme. 5. An inverse dose effect relationship between injected CaNa2EDTA and residual serum dopamine-beta-hydroxylase activity was observed. 6. In spite of the reported lack of changes in copper serum concentration, CaNa2EDTA significantly affects the activity of a copper-dependent enzyme in the serum. 7. As CaNa2EDTA is currently the drug of choice for the treatment of asymptomatic lead poisoning in children, the effect of CaNa2EDTA on copper-enzymes requires further investigation.

摘要

在人体中，依地酸钙钠可增加铅的尿排泄量，同时也会增加锌、铁和锰等必需金属的尿排泄量，但不会增加铜的尿排泄量。2. 我们研究了对三名因疑似铅中毒住院的男性铅焊工注射依地酸钙钠后，对血清多巴胺-β-羟化酶（一种铜依赖性酶）的影响。3. 静脉注射依地酸钙钠（1000毫克）导致血清多巴胺-β-羟化酶活性迅速降低，该活性在24小时内恢复到正常水平。4. 硫酸铜（8微克）而非乙基马来酰亚胺（10毫摩尔）可使体外血清多巴胺-β-羟化酶活性恢复到给药前的值，这表明依地酸钙钠通过从该酶中去除铜来抑制多巴胺-β-羟化酶。5. 观察到注射的依地酸钙钠与残余血清多巴胺-β-羟化酶活性之间存在剂量效应反比关系。6. 尽管报告称血清铜浓度没有变化，但依地酸钙钠会显著影响血清中一种铜依赖性酶的活性。7. 由于依地酸钙钠目前是治疗儿童无症状铅中毒的首选药物，依地酸钙钠对铜酶的影响需要进一步研究。