Martin H
Institut für Pathologie, Paritätisches Krankenhaus Berlin-Lichtenberg (Bereich Oskar-Ziethen-Krankenhaus), Deutschland.
Zentralbl Pathol. 1994 Mar;140(1):15-22.
For the assessment of proliferative activity of malignant tumors several methods can be used. The method having been used at first has been the determination of mitotic index, playing an important role as a part of various grading systems until now. Besides of DNA cytometry, flow cytometry, determination of bromodeoxyuridine labeling index (BrdUr-LI) and immunohistochemical analysis of proliferation-associated antigens (e.g. Ki-67, PCNA), for several years AgNOR analysis has been used as a new technique, giving reproducible, exact results of proliferative activity of malignant tumors. Valuable information about proliferative activity of cells can be gained already by counting the AgNOR number per nucleus. Moreover the use of digital image analysis provides information about size and distribution of AgNORs. Evidently these data are more important than AgNOR number. In the hands of experienced pathologists and under standardized conditions AgNOR staining and AgNOR quantification are a valuable completion of established methods.
对于恶性肿瘤增殖活性的评估，可以使用多种方法。最初使用的方法是有丝分裂指数的测定，直到现在它在各种分级系统中都起着重要作用。除了DNA细胞计数法、流式细胞术、溴脱氧尿苷标记指数（BrdUr-LI）的测定以及增殖相关抗原（如Ki-67、PCNA）的免疫组织化学分析外，多年来，核仁组成区嗜银蛋白（AgNOR）分析一直作为一种新技术使用，可给出恶性肿瘤增殖活性的可重复、准确结果。通过计算每个细胞核中的AgNOR数量，已经可以获得有关细胞增殖活性的有价值信息。此外，数字图像分析的使用提供了有关AgNOR大小和分布的信息。显然，这些数据比AgNOR数量更重要。在经验丰富的病理学家手中并在标准化条件下，AgNOR染色和AgNOR定量是对现有方法的有价值补充。
