Saalbach G, Christov V
Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Plant Mol Biol. 1994 Mar;24(6):969-72. doi: 10.1007/BF00014451.
A clone obtained from a broad bean (Vicia faba) developing cotyledon cDNA library contained the complete coding sequence of a polypeptide with very high homology to the small GTP-binding proteins Ran from human cells and Spi1 from yeast. These proteins belong to the ras superfamily of proteins involved in different basic cellular processes. The Ran/Spi1 proteins interact with a protein bound to DNA (RCC1) and are thought to function in the regulation of the cell cycle. The amino acid sequence of the obtained plant Ran-homologue, designated Vfa-ran, is 74% and 76% identical to Ran and Spi1, respectively. The five functional, conserved domains of ras-related proteins are present in the Vfa-ran sequence. However, as in Ran/Spi1 the C-terminus of Vfa-ran is very acidic and lacks the Cys motif for isoprenylation. Northern blotting revealed a corresponding mRNA expression in broad bean roots, leaves, and cotyledons with the highest level in roots.
从蚕豆（Vicia faba）发育中的子叶cDNA文库获得的一个克隆，包含一种多肽的完整编码序列，该多肽与人类细胞中的小GTP结合蛋白Ran以及酵母中的Spi1具有很高的同源性。这些蛋白质属于参与不同基本细胞过程的ras蛋白超家族。Ran/Spi1蛋白与一种与DNA结合的蛋白（RCC1）相互作用，并被认为在细胞周期调控中发挥作用。所获得的植物Ran同源物（命名为Vfa-ran）的氨基酸序列与Ran和Spi1的分别具有74%和76%的同一性。Vfa-ran序列中存在ras相关蛋白的五个功能保守结构域。然而，与Ran/Spi1一样，Vfa-ran的C末端非常酸性，并且缺乏异戊二烯化的半胱氨酸基序。Northern印迹分析显示蚕豆根、叶和子叶中有相应的mRNA表达，其中根中的表达水平最高。
