Bosco M C, Espinoza-Delgado I, Schwabe M, Russell S M, Leonard W J, Longo D L, Varesio L
Laboratory of Experimental Immunology, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702-1201.
Blood. 1994 Jun 15;83(12):3462-7.
The interleukin-2 receptor gamma (IL-2R gamma) chain is a newly recognized component of the IL-2R of lymphoid cells that is required for their response to IL-2. We investigated the expression of IL-2R gamma protein in human monocytes by Western blot analysis using an antiserum specific for IL-2R gamma. We found that IL-2R gamma subunit is constitutively expressed in human monocytes and upregulated by the monocyte-activating factors IL-2 and interferon gamma (IFN gamma). Furthermore, we show that transforming growth factor beta 1 (TGF beta 1) downmodulates, in a dose-dependent manner, basal and IL-2-induced, but not IFN gamma-induced, IL-2R gamma chain expression, and this effect may be responsible for TGF beta 1 suppressive activity on IL-2-activated monocytes. Overall, these results show that the expression of the IL-2R gamma subunit in human monocytes is tightly regulated by the cytokine network, suggesting a critical role played by this protein on monocyte activation.
白细胞介素-2受体γ（IL-2Rγ）链是淋巴细胞IL-2受体新发现的一个组成部分，淋巴细胞对IL-2作出反应时需要该链。我们使用针对IL-2Rγ的抗血清，通过蛋白质免疫印迹分析研究了人单核细胞中IL-2Rγ蛋白的表达。我们发现IL-2Rγ亚基在人单核细胞中组成性表达，并被单核细胞激活因子IL-2和干扰素γ（IFNγ）上调。此外，我们表明转化生长因子β1（TGFβ1）以剂量依赖的方式下调基础的以及IL-2诱导的，但不是IFNγ诱导的IL-2Rγ链表达，并且这种效应可能是TGFβ1对IL-2激活的单核细胞具有抑制活性的原因。总体而言，这些结果表明人单核细胞中IL-2Rγ亚基的表达受到细胞因子网络的严格调控，提示该蛋白在单核细胞激活中发挥关键作用。
