放射免疫分析法与单向放射免疫扩散法测定血清IgE的比较。

Comparison of serum IgE determination by radioimmunoassay and by single radial immunodiffusion.

作者信息

Berrens L, Bruynzeel P

出版信息

Clin Chim Acta. 1976 Aug 2;70(3):337-42. doi: 10.1016/0009-8981(76)90345-4.

PMID:820496

Abstract

Two commercially available methods of total serum IgE determination have been evaluated, viz: the radioimmunosorbent test (RIST) and the assay by single radial immunodiffusion (RID). RIST was found a suitable and rapid method for the wide range of IgE concentrations to be expected in the sera of an allergic population. The RID method has a lower limit of detection of about 1000 I.U./ml; over this value, both techniques provided statistically correlated results. However, for technological reasons RID was considered less suitable for routine application.

摘要

已对两种市售的总血清IgE测定方法进行了评估，即：放射免疫吸附试验（RIST）和单向放射免疫扩散法（RID）。RIST被发现是一种适用于过敏人群血清中预期的广泛IgE浓度范围的快速方法。RID方法的检测下限约为1000 I.U./ml；超过此值，两种技术提供的结果在统计学上具有相关性。然而，由于技术原因，RID被认为不太适合常规应用。