Kristensen K, Angelo H R, Blemmer T
Royal Danish School of Pharmacy, Department of Pharmaceutics, Universitetsparken 3, Copenhagen.
J Chromatogr A. 1994 Apr 22;666(1-2):283-7. doi: 10.1016/0021-9673(94)80390-0.
A simple and sensitive HPLC method with ultraviolet absorption detection at 200 nm is described for the determination of methadone enantiomers in human serum, using dextropropoxyphene as an internal standard and organic solvent extraction. Separation was performed on two serially coupled columns, CN and Chiral AGP, with a mobile phase consisting of acetonitrile, dimethylocytlamine and phosphate buffer. Using 1.0 ml of serum, 5 nmol/1 of each enantiomer could be determined with an acceptable precision. No interactions from several drugs were observed. The method has been successfully used in a pharmacokinetic study. More than 2500 serum samples have been separated on the same AGP column with acceptable selectivity and resolution.
描述了一种简单且灵敏的高效液相色谱法，用于测定人血清中的美沙酮对映体，采用右丙氧芬作为内标，通过有机溶剂萃取，并在200 nm处进行紫外吸收检测。在两根串联的CN柱和手性AGP柱上进行分离，流动相由乙腈、二甲基乙酰胺和磷酸盐缓冲液组成。使用1.0 ml血清，可测定每种对映体5 nmol/1，精密度可接受。未观察到几种药物之间的相互作用。该方法已成功用于一项药代动力学研究。在同一根AGP柱上已分离了超过2500份血清样品，选择性和分离度均可接受。
