Farthing D, Sica D A, Fakhry I, Gehr T W
Division of Clinical Pharmacology, Medical College of Virginia, Richmond 23298-0160.
J Chromatogr B Biomed Appl. 1994 Mar 4;653(2):171-6. doi: 10.1016/0378-4347(93)e0427-r.
A novel solid-phase on-line elution HPLC method employing fluorescence detection to measure metolazone in plasma and whole blood has been developed. The method is sensitive and selective for metolazone and linear over a dynamic range of 1-50 ng/ml with a sample requirement of 250 microliters. The limit of quantitation for the method is 1 ng/ml and combined intra- and inter-day accuracy and precision had an error and coefficient of variation of 2.9 and 5.5%, respectively.
已开发出一种采用荧光检测的新型固相在线洗脱高效液相色谱法，用于测定血浆和全血中的美托拉宗。该方法对美托拉宗灵敏且具选择性，在1 - 50 ng/ml的动态范围内呈线性，样品需求量为250微升。该方法的定量限为1 ng/ml，日内和日间综合准确度和精密度的误差和变异系数分别为2.9%和5.5%。
