使用焦碳酸二乙酯从动物细胞中纯化RNA。
Purification of RNA from animal cells using diethyl-pyrocarbonate.
作者信息
Tsuei D, Rouhandeh H
出版信息
Experientia. 1976 Jun 15;32(6):749-51. doi: 10.1007/BF01919868.
PMID:820571
Abstract
Extraction of RNA from animal cells by a method using diethyl-pyrocarbonate yielded 50-60% of the total RNA. RNA purified by a hot phenol-SDS method from adenovirus 2 infected cells showed about 9% homology with adenovirus DNA, and RNA purified by diethyl-pyrocarbonate-SDS showed over 7% hybridization. Profiles of RNA prepared by both methods were identical when studied by polyacrylamide gel electrophoresis.
摘要
通过使用焦碳酸二乙酯的方法从动物细胞中提取RNA，得到了总RNA的50 - 60%。用热酚 - SDS法从腺病毒2感染的细胞中纯化的RNA与腺病毒DNA显示出约9%的同源性，而用焦碳酸二乙酯 - SDS法纯化的RNA显示出超过7%的杂交。当通过聚丙烯酰胺凝胶电泳研究时，两种方法制备的RNA图谱是相同的。
Zotero 插件