Keinänen K, Laukkanen M L
VTT Biotechnology and Food Research, Espoo, Finland.
FEBS Lett. 1994 Jun 6;346(1):123-6. doi: 10.1016/0014-5793(94)00313-0.
Chemical conjugation of fatty acids to antibodies generates lipid-modified molecules which have found use in the targeting of liposome-mediated drug delivery and in liposome-based immunoassays. Alternatively, bacterial expression of antibodies as single-chain FV fragments fused to lipoprotein signal peptide and N-terminal sequence leads to in vivo enzymatic addition of a single glycerolipid group at the N-terminus of the molecule. This lipid-modification converts the antibody from a soluble protein into a functional membrane-bound molecule. These biosynthetically lipid-tagged antibodies may prove useful for immobilization of antibodies to membranes in various biotechnological applications.
脂肪酸与抗体的化学偶联产生脂质修饰分子，这些分子已用于脂质体介导的药物递送靶向和基于脂质体的免疫测定。另外，将抗体作为与脂蛋白信号肽和N端序列融合的单链FV片段进行细菌表达，会导致在分子的N端体内酶促添加单个甘油脂基团。这种脂质修饰将抗体从可溶性蛋白质转变为功能性膜结合分子。这些通过生物合成进行脂质标记的抗体可能在各种生物技术应用中用于将抗体固定到膜上。
