Hostálek Z, Tobek I, Bobyk M A, Kulayev I S
Folia Microbiol (Praha). 1976;21(2):131-8. doi: 10.1007/BF02876980.
The activity of ATP-glucokinase and of polyphosphate glucokinase was examined during growth of the actinomycete Streptomyces aureofaciens 8425 under conditions of intense chlortetracycline (CTC) synthesis. ATP-glucokinase was active in the strain only during the logarithmic phase of culture growth; the activity of polyphosphate glucokinase appears only at the end of the logarithmic phase of growth and rises in parallel with the rate of CTC biosynthesis in the stationary phase. During the rise of activity of polyphosphate glucokinase and of CTC biosynthesis the cells accumulate sugar phosphates, mainly glucose-6-phosphate. It appears that the biosynthesis of CTC in Streptomyces aureofaciens takes place at the expense of glycolysis, using up the high-energy phosphate of high-molecular polyphosphates.
在金霉素（CTC）大量合成的条件下，对金色链霉菌8425生长过程中ATP - 葡萄糖激酶和多聚磷酸葡萄糖激酶的活性进行了检测。ATP - 葡萄糖激酶仅在培养物生长的对数期在该菌株中具有活性；多聚磷酸葡萄糖激酶的活性仅在生长对数期结束时出现，并在稳定期与CTC生物合成速率平行上升。在多聚磷酸葡萄糖激酶活性和CTC生物合成增加的过程中，细胞积累磷酸糖，主要是6 - 磷酸葡萄糖。看来金色链霉菌中CTC的生物合成是以糖酵解为代价进行的，消耗了高分子多聚磷酸的高能磷酸键。
