Curdová E, Kremen A, Vanĕk Z, Hostálek Z
Folia Microbiol (Praha). 1976;21(6):481-7. doi: 10.1007/BF02876940.
The relationship was studied between the energy metabolism of the actinomycete Streptomyces aureofaciens and the biosynthesis of chlorotetracycline by this organism. The energy charge values in a culture of low-production strain were almost identical with those of a production variant but the total sum of adenylates was about 10 times higher. In the stationary growth phase both strains evinced a drop in energy charge values followed by a rise to the original level. An increase in the concentration of inorganic phosphate in fermentation medium caused a suppression of antibiotic formation in the lowproduction strain and further rise in the total adenylate level. The expression of the energy charge in Streptomyces aureofaciens acquires a complex character owing to the participation, apart from the adenylate system, of high-molecular polyphosphates as energy donors and the probable lack of a regulating mechanism such as the adenylate kinase reaction.
研究了金色链霉菌的能量代谢与其四环素生物合成之间的关系。低产菌株培养物中的能荷值与高产变异株的几乎相同，但腺苷酸的总量高出约10倍。在稳定生长期，两种菌株的能荷值均下降，随后又回升至原始水平。发酵培养基中无机磷酸盐浓度的增加导致低产菌株中抗生素形成受到抑制，腺苷酸总量进一步升高。由于除腺苷酸系统外，高分子多磷酸盐作为能量供体的参与以及可能缺乏诸如腺苷酸激酶反应等调节机制，金色链霉菌中能荷的表达呈现出复杂的特征。
