Eisemann C S, Osterman J V
Infect Immun. 1976 Jul;14(1):155-62. doi: 10.1128/iai.14.1.155-162.1976.
Purified radioactive rickettsiae were obtained from irradiated and cycloheximide-inhibited L cells, and their proteins were analyzed by polyacrylamide gel electrophoresis. Rickettsial species could be distinguished by comparing the relative mobilities of constituent proteins after migration of two differentially labeled preparations in a single gel. Distinct differences were observed in gel patterns of rickettsiae from the typhus and spotted fever groups, as well as with different species within a group. Rickettsial organisms causing murine and epidemic typhus were clearly distinguished, as were the causative agentsof boutonneuse fever and rickettsialpox. The use of both internal and external molecular weight standards allowed molecular weight estimates for 19 proteins from both Rickettsia prowazekii and Rickettsia conorii. A flexible system for designating rickettsial proteins is proposed that lends itself to modification as more detailed analysis progresses.
从经辐照和环己酰亚胺抑制的L细胞中获得纯化的放射性立克次氏体，并通过聚丙烯酰胺凝胶电泳分析其蛋白质。通过比较两种差异标记制剂在单一凝胶中迁移后组成蛋白质的相对迁移率，可以区分立克次氏体种类。在斑疹伤寒组和斑点热组的立克次氏体凝胶图谱中观察到明显差异，同一组内不同种之间也存在差异。引起鼠型斑疹伤寒和流行性斑疹伤寒的立克次氏体微生物以及纽扣热和立克次体痘的病原体都能被清楚地区分。使用内部和外部分子量标准可以估计普氏立克次氏体和康氏立克次氏体中19种蛋白质的分子量。提出了一种灵活的立克次氏体蛋白质命名系统，随着更详细的分析进展，该系统便于修改。
