Joly E, Deverson E V, Coadwell J W, Günther E, Howard J C, Butcher G W
Department of Immunology, Babraham Institute, Cambridge, UK.
Immunogenetics. 1994;40(1):45-53. doi: 10.1007/BF00163963.
We are reporting the cDNA sequences of Tap2 from two cima and two cimb rat strains. Comparison of the cDNA sequences shows that these alleles fall into two groups, which we refer to as Tap2-A and Tap2-B. We found that alleles from the Tap2-B group are more closely related to the mouse homologue than are Tap2-A alleles, and among the 48 nucleotides which differ between the Tap2-A and Tap2-B cDNAs, three affect restriction sites. We defined pairs of oligonucleotides which allow amplification of the regions bearing these restriction sites from genomic DNA or cDNA, and this technique has been successful for the genotyping of all of the 56 laboratory strains of Rattus norvegicus tested and for five cell lines tested so far. All 14 known RT1 standard haplotypes were tested, and 7 found to belong to the Tap2-B group, and 7 to Tap2-A. We also found that intron sizes among the alleles of the Tap2-B group fall into two subgroups, providing further insight into the phylogeny of these various haplotypes.
我们报告了来自两种cima和两种cimb大鼠品系的Tap2的cDNA序列。对cDNA序列的比较表明，这些等位基因分为两组，我们将其称为Tap2 - A和Tap2 - B。我们发现，Tap2 - B组的等位基因比Tap2 - A等位基因与小鼠同源物的关系更密切，并且在Tap2 - A和Tap2 - B cDNA之间不同的48个核苷酸中，有三个影响限制性酶切位点。我们定义了几对寡核苷酸，可用于从基因组DNA或cDNA中扩增带有这些限制性酶切位点的区域，并且该技术已成功用于对所测试的56种挪威大鼠实验室品系以及目前测试的5种细胞系进行基因分型。对所有14种已知的RT1标准单倍型进行了测试，发现7种属于Tap2 - B组，7种属于Tap2 - A组。我们还发现，Tap2 - B组等位基因的内含子大小分为两个亚组，这为深入了解这些不同单倍型的系统发育提供了进一步的线索。
