Powis S J, Howard J C, Butcher G W
Department of Immunology, Agricultural & Food Research Council, Institute of Animal Physiology & Genetics Research, Cambridge Research Station, Babraham, UK.
J Exp Med. 1991 Apr 1;173(4):913-21. doi: 10.1084/jem.173.4.913.
The dominant trans-acting major histocompatibility complex (MHC)-linked class I modifier (cim) locus, previously recognized through its ability to determine altered alloantigenicity of a rat class I molecule, RT1.A3, is shown here to influence class I intracellular transport. The MHC recombinant laboratory rat strains PVG.R1 and PVG.R8 display unusually long retention of RT1.Aa within the endoplasmic reticulum or cis-Golgi. In appropriate F1 hybrid cells heterozygous for RT1.Aa and another class I MHC allele, RT1.Ac, only the RT1.Aa protein is subject to slow transport. The cim gene product therefore shows class I allele specificity in its action, cim appears to be a polymorphic locus whose product is directly involved in the processes of class I MHC assembly and/or intracellular transport.
主要的反式作用主要组织相容性复合体(MHC)连锁的I类修饰基因(cim)位点,先前通过其决定大鼠I类分子RT1.A3改变的同种抗原性的能力而被识别,此处显示其影响I类分子的细胞内运输。MHC重组实验大鼠品系PVG.R1和PVG.R8在粗面内质网或顺式高尔基体中显示RT1.Aa异常长时间的滞留。在对RT1.Aa和另一个I类MHC等位基因RT1.Ac杂合的合适F1杂交细胞中,只有RT1.Aa蛋白运输缓慢。因此,cim基因产物在其作用中显示I类等位基因特异性,cim似乎是一个多态性位点,其产物直接参与I类MHC组装和/或细胞内运输过程。