Herbergs J, de Bruïne A P, Marx P T, Vallinga M I, Stockbrügger R W, Ramaekers F C, Arends J W, Hopman A H
Department of Pathology, University Hospital, Maastricht, The Netherlands.
Int J Cancer. 1994 Jun 15;57(6):781-5. doi: 10.1002/ijc.2910570604.
Thirty-five colon adenomas from 26 patients were analyzed with centromeric probes for chromosomes 1, 7, 17, X and Y in order to study numerical aberrations, chromosome imbalances, aneuploidy and tetraploidization. The fluorescent in situ hybridization (FISH) technique was applied to single-cell suspensions and a combination of FISH and immunocytochemistry (ICC) was employed to identify the cell type under study. Trisomy of chromosome 7 was detected in 37% of the cases. In 7 out of 13 cases this aberration was combined with abnormalities of one or 2 of the other investigated chromosomes. No correlation could be demonstrated between any of the detected chromosomal aberrations and size, localization or degree of epithelial dysplasia. With the combined FISH/ICC procedure, the abnormal cells were shown to be of epithelial rather than of stromal origin. Our data indicate that trisomy 7 is a common chromosome aberration in the epithelial component of colon adenomas.
为了研究数目畸变、染色体失衡、非整倍体和四倍体化，对来自26名患者的35个结肠腺瘤用1、7、17、X和Y染色体的着丝粒探针进行了分析。荧光原位杂交（FISH）技术应用于单细胞悬液，并且采用FISH与免疫细胞化学（ICC）相结合的方法来识别所研究的细胞类型。在37%的病例中检测到7号染色体三体。在13例中的7例中，这种畸变与其他一条或两条被研究染色体的异常同时存在。在所检测到的任何染色体畸变与大小、定位或上皮发育异常程度之间均未显示出相关性。通过FISH/ICC联合程序，显示异常细胞来源于上皮而非间质。我们的数据表明，7号染色体三体是结肠腺瘤上皮成分中常见的染色体畸变。
