Nanashima A, Tagawa Y, Yasutake T, Sawai T, Tuji T, Sasano O, Nakagoe T, Ayabe H
First Department of Surgery, Nagasaki University School of Medicine, Japan.
J Gastroenterol. 1997 Aug;32(4):487-91. doi: 10.1007/BF02934087.
Specific loss of heterozygosity of chromosome 18 has been observed frequently in advanced colorectal carcinoma and is closely associated with its development. We investigated the prevalence of numerical aberrations of chromosome 18 in 44 specimens of colorectal carcinomas, using fluorescence in situ hybridization. We also examined the relationship between aneusomy of chromosome 18 and the clinicopathological features of these tumors. Aneusomy of the specimens (monosomy and polysomy) was determined when the same aneusomic population was detected in more than 15% of the nuclei. The frequency of monosomy and polysomy of chromosome 18 in colorectal carcinomas was 43% (19/44) and 29% (12/44), respectively. The prevalence of monosomy and polysomy 18 was significantly higher in cancers with invasion exceeding category T2 compared with T1 (P < 0.01), and with tumor size exceeding 20 mm in diameter compared with tumors less than 20 mm (P < 0.05). However, the prevalence of aneusomy 18 was not associated with other clinico-pathological features. The mean survival period and the 5-year survival rate after operation in patients with aneusomy 18 was not different from findings for those with disomy 18. Our results indicate that aneusomy of chromosome 18 is associated with the development of colorectal carcinoma; however, it is not a useful indicator of postoperative prognosis.
在进展期结直肠癌中经常观察到18号染色体杂合性的特异性缺失，且这与其发展密切相关。我们使用荧光原位杂交技术研究了44例结直肠癌标本中18号染色体数目畸变的发生率。我们还研究了18号染色体非整倍体与这些肿瘤临床病理特征之间的关系。当在超过15%的细胞核中检测到相同的非整倍体群体时，确定标本的非整倍体（单体和多体）情况。结直肠癌中18号染色体单体和多体的频率分别为43%（19/44）和29%（12/44）。与T1期癌症相比，浸润超过T2期的癌症中18号染色体单体和多体的发生率显著更高（P < 0.01）；与直径小于20 mm的肿瘤相比，直径超过20 mm的肿瘤中18号染色体单体和多体的发生率显著更高（P < 0.05）。然而，18号染色体非整倍体的发生率与其他临床病理特征无关。18号染色体非整倍体患者术后的平均生存期和5年生存率与18号染色体二倍体患者的结果无差异。我们的结果表明，18号染色体非整倍体与结直肠癌的发展相关；然而，它不是术后预后的有用指标。
