Jagetia G C, Jacob P S
Department of Radiobiology, Kasturba Medical College, Manipal, India.
Hereditas. 1994;120(1):51-9. doi: 10.1111/j.1601-5223.1994.00051.x.
The combined effects of vinblastine sulphate (VBL) and gamma radiation treatments on the induction of micronuclei in polychromatic erythrocytes (MPCE) and normochromatic erythrocytes (MNCE) and changes in polychromatic/normochromatic erythrocyte ratio (P/N ratio) in mouse bone marrow, were studied. Compared to double distilled water (DDW) injected controls, 0.05 mg/kg b.wt. of vinblastine alone significantly increased the frequency of MPCE and MNCE. When VBL was administered before exposure to various doses of gamma radiation the frequency of micronuclei (MPCE and MNCE) increased with the increasing radiation dose up to a dose of 4 Gy, but this increase levelled off at 3 to 4 Gy irradiation. The frequency of MPCE was found to be significantly lower at 3 and 4 Gy radiation when compared to the DDW+irradiated group. A significant dose dependent decline in the P/N ratio was observed in the DDW+irradiated group. VBL treatment before irradiation resulted in a more significant decline in the P/N ratio than that of DDW+irradiated group. The formation of micronuclei (MPCE and MNCE) increased from 8 h post-exposure and continued to increase up to 28 h. There was a sharp decline in the frequency of micronuclei (MPCE and MNCE) at 32 h post-irradiation, in both DDW + 1 Gy irradiated and VBL + 1 Gy irradiated groups; then it declined gradually up to 72 h post-exposure without restoration to normal level. The P/N ratio declined with time in all three groups (VBL alone, DDW+irradiation, and VBL+irradiation) without restoration to normal level up to 72 h post-treatment, except in the DDW+irradiated group, where it was normal.
研究了硫酸长春碱（VBL）与γ射线联合处理对小鼠骨髓中多染性红细胞（MPCE）和正染性红细胞（MNCE）微核诱导以及多染性/正染性红细胞比率（P/N比率）变化的影响。与注射双蒸水（DDW）的对照组相比，单独使用0.05 mg/kg体重的长春碱可显著增加MPCE和MNCE的频率。当在暴露于不同剂量的γ射线之前给予VBL时，微核（MPCE和MNCE）频率随辐射剂量增加而增加，直至4 Gy剂量，但在3至4 Gy照射时这种增加趋于平稳。与DDW+照射组相比，在3 Gy和4 Gy辐射时MPCE频率显著降低。在DDW+照射组中观察到P/N比率有显著的剂量依赖性下降。照射前VBL处理导致P/N比率下降比DDW+照射组更显著。微核（MPCE和MNCE）的形成从暴露后8小时开始增加，并持续增加至28小时。在DDW + 1 Gy照射组和VBL + 1 Gy照射组中，照射后32小时微核（MPCE和MNCE）频率均急剧下降；然后在暴露后72小时逐渐下降，未恢复到正常水平。在所有三组（单独VBL、DDW+照射和VBL+照射）中，P/N比率均随时间下降，直至处理后72小时未恢复到正常水平，但DDW+照射组除外，该组P/N比率正常。
