Jagetia G C, Nayak V
Department of Radiobiology, Kasturba Medical College, Manipal 576 119 India.
Mutat Res. 1996 Feb 1;349(2):219-27. doi: 10.1016/0027-5107(95)00181-6.
The frequency of micronucleated polychromatic erythrocytes (MPCE) and the normochromatic erythrocytes (MNCE) and polychromatic and normochromatic erythrocyte ratio (P/N ratio) was studied at 12, 24 and 36 h postirradiation in the bone marrow of male mice treated or not with taxol before exposure to 0-4 Gy of 60Co gamma radiation. The frequency of MPCE increased with the increase in radiation dose in a dose-related manner in the irradiated control group. A peak frequency of MPCE was observed at 24 h postirradiation in irradiated control group. The pattern of increase in MNCE was similar to that of MPCE except that a highest number of MNCE was scored at 36 h postirradiation. Taxol administration to animals before irradiation resulted in a significant elevation in the frequency of MPCE and MNCE at all the postirradiation time periods studied. This increase was dose related as observed in the irradiated control group. Irradiation resulted in a dose-dependent decline in the P/N ratio at all the postirradiation time periods studied. The P/N ratio was significantly lower in the taxol + irradiated group compared to the irradiated control group at all postirradiation time periods. A maximum decline in P/N ratio was observed at 36 h postirradiation for both irradiated control and taxol + irradiated groups. The dose response for MPCE, MNCE and P/N ratio was linear quadratic for both the irradiated and taxol + irradiated groups.
在雄性小鼠暴露于0 - 4 Gy的60Coγ射线之前，用或不用紫杉醇处理，然后在照射后12、24和36小时研究骨髓中微核多色红细胞（MPCE）、正常色红细胞（MNCE）的频率以及多色与正常色红细胞比率（P/N比率）。在照射对照组中，MPCE的频率随辐射剂量增加呈剂量相关方式增加。照射对照组在照射后24小时观察到MPCE的频率峰值。MNCE的增加模式与MPCE相似，只是在照射后36小时记录到最高数量的MNCE。在照射前给动物施用紫杉醇导致在所有研究的照射后时间段内MPCE和MNCE的频率显著升高。如在照射对照组中观察到的那样，这种增加与剂量相关。在所有研究的照射后时间段内，照射导致P/N比率呈剂量依赖性下降。在所有照射后时间段，紫杉醇 + 照射组的P/N比率显著低于照射对照组。照射对照组和紫杉醇 + 照射组在照射后36小时均观察到P/N比率的最大下降。照射组和紫杉醇 + 照射组的MPCE、MNCE和P/N比率的剂量反应均为线性二次关系。
