Radder A M, Leenders H, van Blitterswijk C A
Laboratory for Otobiology and Biocompatibility, University of Leiden, The Netherlands.
J Biomed Mater Res. 1994 Feb;28(2):141-51. doi: 10.1002/jbm.820280202.
The bone reaction at the interface of a range of PEO/PBT copolymers (Polyactive) after press-fit implantation in the diaphyseal femur of 20 goats was investigated. The animals were sacrificed at 1, 4, 12, 26, and 52 weeks. Undecalcified histologic sections were prepared, evaluated qualitatively, and quantified with the aid of an image analysis system. The percentage of bone contact was determined morphometrically for all PEO/PBT proportions; for the 70/30 ratio, the percentage of calcification of the material surface and of bone-bonding (defined as a continuum at the light microscopic level between calcification within the material surface and bone tissue) were also assessed. PEO/PBT copolymers possess hydrogelic properties, and a direct relation has been established between water-uptake and PEO content. The swelling behavior of all PEO/PBT substrates resulted in a high degree of bone contact (over 95%) at early stages. From the 12-week survival time onward the 70/30 revealed higher percentages of bone contact (over 80%) when compared with the other ratios (60/40, 55/45, 40/60, and 30/70). This difference was statistically confirmed 1 year postoperatively. The materials with the higher PEO contents showed the highest amounts of calcification, which is reflected by the rate of bone-bonding (70% of bone-bonding for the 70/30 at 52 weeks). Calcification and bone-bonding were not observed for 40/60 and 30/70 proportions. The quantitative results confirmed the relation between PEO content, water-uptake, calcification, and bone-bonding. It was furthermore demonstrated that PEO/PBT copolymers initially establish a high degree of bone contact, whereas the bone-bonding properties, especially of the 70/30, accomplish a long-term implant fixation.
研究了一系列聚环氧乙烷/聚对苯二甲酸丁二醇酯共聚物（Polyactive）在20只山羊股骨干骺端压配植入后的骨界面反应。在1、4、12、26和52周时对动物实施安乐死。制备未脱钙组织学切片，进行定性评估，并借助图像分析系统进行定量分析。测定了所有聚环氧乙烷/聚对苯二甲酸丁二醇酯比例下的骨接触百分比；对于70/30比例，还评估了材料表面钙化和骨结合（在光学显微镜水平定义为材料表面钙化与骨组织之间的连续体）的百分比。聚环氧乙烷/聚对苯二甲酸丁二醇酯共聚物具有水凝胶特性，且已确定吸水率与聚环氧乙烷含量之间存在直接关系。所有聚环氧乙烷/聚对苯二甲酸丁二醇酯基质的肿胀行为导致早期骨接触程度较高（超过95%）。从12周存活期起，与其他比例（60/40、55/45、40/60和30/70）相比，70/30比例显示出更高的骨接触百分比（超过80%）。术后1年，这种差异得到统计学证实。聚环氧乙烷含量较高的材料显示出最高的钙化量，这通过骨结合率得以体现（52周时70/30比例的骨结合率为70%）。对于40/60和30/70比例，未观察到钙化和骨结合。定量结果证实了聚环氧乙烷含量、吸水率、钙化和骨结合之间的关系。此外还表明，聚环氧乙烷/聚对苯二甲酸丁二醇酯共聚物最初建立了高度的骨接触，而骨结合特性，尤其是70/30比例的骨结合特性，实现了长期的植入物固定。
