Mabondzo A, Boussin F, Raoul H, Le Naour R, Gras G, Vaslin B, Bartholeyns J, Romet-Lemonne J L, Dormont D
Laboratoire de Neuropathologie expérimentale et Neurovirologie, CRSSA, DSV/DPTE, Commissariat à l'Energie Atomique, Fontenay-aux-Roses, France.
J Gen Virol. 1994 Jun;75 ( Pt 6):1451-6. doi: 10.1099/0022-1317-75-6-1451.
Human monocytes/macrophages, which express Fc receptors for IgG are involved in human immunodeficiency virus type 1 (HIV-1) infection and pathogenesis. These receptors are known to mediate numerous immunological functions including cell-mediated killing and possibly targeting of HIV to the lysophagosome monocyte-derived macrophage (MDM) entry route for virus neutralization. To study both activities in HIV-1 infection, MDM Fc gamma RI was specifically selected using bispecific antibody (Bs-Ab) containing whole human monoclonal antibody against gp41 and the Fab' fragment of murine anti-Fc gamma RI 22.2 antibody. Bs-Ab was found to mediate potent antibody-dependent cellular cytotoxicity and virus neutralization.
表达IgG Fc受体的人单核细胞/巨噬细胞参与了1型人类免疫缺陷病毒（HIV-1）的感染和发病机制。已知这些受体介导多种免疫功能，包括细胞介导的杀伤作用，以及可能将HIV靶向至溶酶体-吞噬体，这是单核细胞衍生巨噬细胞（MDM）的病毒中和进入途径。为了研究HIV-1感染中的这两种活性，使用含有抗gp41全人单克隆抗体和鼠抗FcγRI 22.2抗体Fab'片段的双特异性抗体（Bs-Ab）特异性选择了MDM FcγRI。发现Bs-Ab介导强效的抗体依赖性细胞毒性和病毒中和作用。
