Kleftogiannis F, Handley C J, Campbell M A
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
J Orthop Res. 1994 May;12(3):365-74. doi: 10.1002/jor.1100120309.
Synovial capsule from the metacarpophalangeal joints of cattle was shown to be a highly collagenous tissue, with a hydroxyproline content of 100 +/- 1 micrograms/mg dry weight and a water content of 70 +/- 3.6%. Type-I collagen made up 83% of the collagen present, and the remainder was type III. When incubated in explant culture, synovial capsule incorporated [3H]acetate into both glycoproteins and hyaluronan and [3H]acetate and [35S]sulfate into proteoglycans. The rate of synthesis of proteoglycans by synovial tissue was shown to be similar to that measured for collateral ligament from the same joint. Two populations of proteoglycans were observed to be synthesized by synovial capsule. More than 90% of the 35S-labelled proteoglycans eluted with a K(av) of 0.7 on Sepharose CL-4B, and the remainder of the radiolabelled macromolecules eluted from the column with a K(av) of less than 0.5. Analysis of the major population of proteoglycans showed it to consist of a dermatan sulfate-containing proteoglycan with a core protein of 45,000 Da that had the same N-terminal amino acid sequence as decorin.
牛掌指关节的滑膜囊被证明是一种富含胶原蛋白的组织，羟脯氨酸含量为100±1微克/毫克干重，含水量为70±3.6%。I型胶原蛋白占现有胶原蛋白的83%，其余为III型。在器官培养中培养时，滑膜囊将[3H]乙酸盐掺入糖蛋白和透明质酸中，并将[3H]乙酸盐和[35S]硫酸盐掺入蛋白聚糖中。滑膜组织中蛋白聚糖的合成速率与同一关节侧副韧带测得的合成速率相似。观察到滑膜囊合成了两种蛋白聚糖群体。超过90%的35S标记的蛋白聚糖在Sepharose CL - 4B上以0.7的K(av)洗脱，其余放射性标记的大分子以小于0.5的K(av)从柱上洗脱。对主要蛋白聚糖群体的分析表明，它由一种含硫酸皮肤素的蛋白聚糖组成，其核心蛋白为45,000 Da，与核心蛋白聚糖具有相同的N端氨基酸序列。
