Louie A, Dixon D M, el-Maghrabi E A, Burnett J W, Baltch A L, Smith R P
Division of Infectious Diseases, Stratton Veterans Affairs Medical Center, Albany, NY 12208.
J Med Vet Mycol. 1994;32(1):59-64.
Five strains of Candida albicans with previously characterized epidermolytic acid proteinase activity were evaluated for virulence following intravenous (i.v.) injection in mice. Increased proteinase activity was associated with increased virulence in female, NYLAR mice receiving 10(6) cells i.v. Mean mortality times (1.25, 2.0, 2.0, 4.25 and 19.6 days, in groups of 20 mice for each of the five strains) correlated directly with degree of proteinase activity. Three of the strains were selected for additional in vivo study and the association between increased proteinase activity and increased mortality rates was confirmed in dose-response studies in two additional strains of mice. The mean survival times appeared to be independent of fungal growth rate in vitro. These results support the positive correlation between proteinase activity and virulence.
对五株先前已鉴定具有表皮溶解酸性蛋白酶活性的白色念珠菌菌株进行了静脉注射（i.v.）小鼠后的毒力评估。在静脉注射10(6)个细胞的雌性NYLAR小鼠中，蛋白酶活性增加与毒力增强相关。平均死亡时间（五株菌株每组20只小鼠，分别为1.25、2.0、2.0、4.25和19.6天）与蛋白酶活性程度直接相关。选择其中三株菌株进行额外的体内研究，并且在另外两株小鼠的剂量反应研究中证实了蛋白酶活性增加与死亡率增加之间的关联。平均存活时间似乎与体外真菌生长速率无关。这些结果支持了蛋白酶活性与毒力之间的正相关关系。
