Valois M E, Takayanagui O M, Bonato P S, Lanchote V L, Carvalho D
Faculty of Pharmacy, Federal University of Maranhão, Brazil.
J Anal Toxicol. 1994 Mar-Apr;18(2):86-90. doi: 10.1093/jat/18.2.86.
Albendazole is an antihelminthic agent belonging to the benzimidazole class and has been used successfully in the treatment of neurocysticercosis. We report here a method for the determination of the two major albendazole metabolites in plasma, albendazole sulfone and albendazole sulfoxide. The method consists of drug extraction from 500 microL of plasma previously acidified with chloroform-isopropanol (9:1, v/v) and extract purification with n-hexane immediately before chromatographic analysis. Separation of drugs and of the internal standard (mebendazole) was performed on an RP-18 column using acetonitrile-0.25N sodium acetate buffer (3:7, v/v), pH 5.0, as the mobile phase and using detection at 290 nm.
阿苯达唑是一种属于苯并咪唑类的抗蠕虫药，已成功用于治疗神经囊尾蚴病。我们在此报告一种测定血浆中两种主要阿苯达唑代谢物阿苯达唑砜和阿苯达唑亚砜的方法。该方法包括先用氯仿 - 异丙醇（9:1，v/v）酸化500微升血浆进行药物提取，然后在色谱分析前立即用正己烷进行提取物纯化。药物和内标（甲苯达唑）在RP - 18柱上进行分离，使用乙腈 - 0.25N醋酸钠缓冲液（3:7，v/v），pH 5.0作为流动相，并在290nm处进行检测。
