Loughran T P, Sherman M P, Ruscetti F W, Frey S, Coyle T, Montagna R A, Jones B, Starkebaum G, Poiesz B J
Veterans Affairs Medical Center, Syracuse, NY 13210.
Leuk Res. 1994 Jun;18(6):423-9. doi: 10.1016/0145-2126(94)90078-7.
The etiology of LGL leukemia is not known; however, we recently detected HTLV-II in a patient with LGL leukemia. In this study, we found that sera from 6 of 28 patients with LGL leukemia were positive for HTLV-I/II using a whole virus ELISA; moreover, the ELISA-negative sera were near the positive cut-off value. Therefore, we performed additional studies on these sera using commercially available assays which can confirm and distinguish HTLV-I from HTLV-II infection. Serum from only one patient was confirmed positive using conventional criteria (HTLV-II+). Sera from 25 patients (89%) had indeterminate reactivity on Western blot assays. Of these, sera from 21 (84%) reacted to gag protein p24; 12 (48%) reacted with recombinant env protein p21e, and 10 (40%) reacted with both. We could not detect HTLV-I/II pol or pX gene sequences in these patients using polymerase chain reaction analyses, with the exception of the HTLV-II-infected patient described previously. These data show that most patients with LGL leukemia are not infected with prototypical HTLV-I or HTLV-II. The frequent reactivity of patient sera to HTLV-I/II gag protein p24 and to env protein p21e, however, suggests that a deleted or variant form of HTLV-I/II may be associated with LGL leukemia.
大颗粒淋巴细胞白血病（LGL白血病）的病因尚不清楚；然而，我们最近在一名LGL白血病患者中检测到了人类嗜T淋巴细胞病毒II型（HTLV-II）。在本研究中，我们发现，使用全病毒酶联免疫吸附测定（ELISA），28例LGL白血病患者中有6例的血清HTLV-I/II呈阳性；此外，ELISA阴性的血清接近阳性临界值。因此，我们使用可用于确认和区分HTLV-I感染与HTLV-II感染的市售检测方法，对这些血清进行了进一步研究。仅1例患者的血清根据传统标准被确认为阳性（HTLV-II+）。25例患者（89%）的血清在蛋白质印迹分析中反应不确定。其中，21例（84%）患者的血清与gag蛋白p24反应；12例（48%）与重组env蛋白p21e反应，10例（40%）与两者均反应。除先前描述的HTLV-II感染患者外，我们使用聚合酶链反应分析在这些患者中未检测到HTLV-I/II pol或pX基因序列。这些数据表明，大多数LGL白血病患者未感染典型的HTLV-I或HTLV-II。然而，患者血清对HTLV-I/II gag蛋白p24和env蛋白p21e的频繁反应表明，HTLV-I/II的缺失或变异形式可能与LGL白血病有关。
