用于确认和区分人类I型和II型嗜T细胞病毒的改良蛋白质印迹法。

Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II.

作者信息

Lipka J J, Santiago P, Chan L, Reyes G R, Samuel K P, Blattner W A, Shaw G M, Hanson C V, Sninsky J J, Foung S K

机构信息

Department of Pathology, Stanford University School of Medicine, Palo Alto, CA.

出版信息

J Infect Dis. 1991 Aug;164(2):400-3. doi: 10.1093/infdis/164.2.400.

DOI:10.1093/infdis/164.2.400

PMID:1856487

Abstract

Disease association studies of human T cell lymphotropic virus (HTLV) types I and II are hindered by the need for multiple assays to confirm and differentiate between the viruses. A modified Western blot assay has been developed using HTLV-I viral lysate and unique (MTA-4) and shared (p21E) HTLV recombinant proteins. By defining confirmation of infection as the presence of antibodies to p24 gag protein and to p21E, all 56 HTLV-I and 49 HTLV-II antisera were confirmed by this modified Western blot alone. Differentiation was determined by reactivity to MTA-4. All HTLV-I antisera reacted with MTA-4 and all HTLV-II antisera did not react with MTA-4. These findings indicate the utility of selected HTLV-I recombinant proteins in a single assay format to confirm and differentiate infections with HTLV-I and HTLV-II.

摘要

人类嗜T细胞病毒（HTLV）I型和II型的疾病关联研究因需要多种检测方法来确认和区分这两种病毒而受到阻碍。利用HTLV-I病毒裂解物以及独特的（MTA-4）和共享的（p21E）HTLV重组蛋白，开发了一种改良的蛋白质印迹检测法。通过将感染确认定义为存在针对p24 gag蛋白和p21E的抗体，仅通过这种改良的蛋白质印迹法就确认了所有56份HTLV-I抗血清和49份HTLV-II抗血清。通过对MTA-4的反应性来确定区分。所有HTLV-I抗血清都与MTA-4反应，而所有HTLV-II抗血清都不与MTA-4反应。这些发现表明，所选的HTLV-I重组蛋白在单一检测形式中可用于确认和区分HTLV-I和HTLV-II感染。