Matulić M, Sorić J
University Hospital for Tumors, Laboratory for Experimental Cancerology, Zagreb, Croatia.
Neoplasma. 1994;41(2):95-100.
Eighty-four biopsies derived from cervical tissues were analyzed for the presence of human papillomavirus (HPV) DNA types 6, 16 and 18 using Southern blot hybridization. HPV 6 was found in none of the cervical biopsies, and HPV types 16 and 18 were found in 44% of them. The rate of HPV 16/18 positive samples increased proportionally to the severity of the lesion. In normal tissue there were no positive samples, in mild and moderate dysplasias HPV 16/18 was present in 20% and in severe dysplasias and invasive carcinomas in 37 and 50%, respectively. In biopsies from 13 cases with squamous cell carcinoma of the uterine cervix and CIN III lesions HPV 16 was integrated within the host genome. It was concluded that the virus could be integrated at variable, presumably randomly selected chromosomal loci and with different number of copies. Transcription of HPV 16 and 18 was detected in one cervical cancer and in HeLa cells, respectively. These results imply that HPV types 16 and 18 play an etiological role in the carcinogenesis of human cervical epithelial cells.
采用Southern印迹杂交法对84份取自宫颈组织的活检样本进行分析，以检测人乳头瘤病毒（HPV）6、16和18型DNA的存在情况。在所有宫颈活检样本中均未发现HPV 6，而HPV 16和18型在44%的样本中被检测到。HPV 16/18阳性样本的比例与病变严重程度成正比。在正常组织中没有阳性样本，在轻度和中度发育异常中，HPV 16/18的存在率为20%，在重度发育异常和浸润性癌中分别为37%和50%。在13例子宫颈鳞状细胞癌和CIN III病变的活检样本中，HPV 16整合到了宿主基因组中。得出的结论是，该病毒可能整合到可变的、大概是随机选择的染色体位点上，且拷贝数不同。分别在1例宫颈癌和HeLa细胞中检测到了HPV 16和18的转录。这些结果表明，HPV 16和18型在人类宫颈上皮细胞癌变过程中起病因学作用。
