通过简化的巢式扩增方案快速诊断结核性脑膜炎。
Rapid diagnosis of tuberculous meningitis by a simplified nested amplification protocol.
作者信息
Liu P Y, Shi Z Y, Lau Y J, Hu B S
机构信息
Department of Internal Medicine, Taichung Veterans General Hospital, Taiwan, R.O.C.
出版信息
Neurology. 1994 Jun;44(6):1161-4. doi: 10.1212/wnl.44.6.1161.
PMID:8208416
Abstract
We have developed a simplified protocol based on nested polymerase chain reaction (PCR) for early diagnosis of tuberculous meningitis. Using this protocol, we detected the Mycobacterium tuberculosis genome within 24 hours in the CSF of 19 of 21 patients (90%) with clinically suspected tuberculous meningitis. The PCR results were negative in all 79 nontuberculous meningitis controls.
摘要
我们基于巢式聚合酶链反应（PCR）开发了一种简化方案，用于结核性脑膜炎的早期诊断。使用该方案，我们在21例临床疑似结核性脑膜炎患者中的19例（90%）脑脊液中24小时内检测到结核分枝杆菌基因组。在所有79例非结核性脑膜炎对照中，PCR结果均为阴性。
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